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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

Plasmid

#159664

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

Plasmid

#159663

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

Plasmid

#159662

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

Plasmid

#159656

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Promoter

Ef1a

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-RPS14(WT)-Myc

Plasmid

#122236

Purpose

expresses RPS14 protein with a Myc tag in mammalian cells (hygro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-RSLID1(WT)

Plasmid

#122296

Purpose

expresses RSL1D1 protein in mammalian cells (puro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RSL1D1 (RSL1D1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

vector's LTR

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL3331 MTK234 hU6-TetO-BtoApegRNA

Plasmid

#254860

Purpose

MTK234 part encoding a doxycycline-inducible B-to-A pegRNA for the peCHYRON system. Can be used for transient transfection or incorporated into larger MTK assemblies.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

TetO_hU6_promoter-peCHYRON_BtoA_pegRNA

Expression

Mammalian

Available Since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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ARL13B-(ΔCC)-GFP

Plasmid

#246844

Purpose

Express Arl13B (resistance to sgRNA) with deletion of coiled-coil region, tagged with GFP

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted the coiled-coil region domain (aa190-245)

Available Since

June 11, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pur-BFP-P2A-otMx1

Plasmid

#224553

Purpose

Expresses Oncorhynchus tshawytscha mx1 protein and BFP

Depositor

Bertrand Collet

Article

Chaumont et al Fish Shellfish Immunol. 2026 Apr;171:111

Insert

BFP-P2A-otMx1 (LOC112247236 Oncorhynchus tshawytscha)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pur-BFP-P2A-otMx2

Plasmid

#224554

Purpose

Expresses Oncorhynchus tshawytscha mx2 protein and BFP

Depositor

Bertrand Collet

Article

Chaumont et al Fish Shellfish Immunol. 2026 Apr;171:111

Insert

BFP-P2A-otMx2 (LOC121839060 Oncorhynchus tshawytscha)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pur-BFP-P2A-otMx3

Plasmid

#224555

Purpose

Expresses Oncorhynchus tshawytscha mx3 protein and BFP

Depositor

Bertrand Collet

Article

Chaumont et al Fish Shellfish Immunol. 2026 Apr;171:111

Insert

BFP-P2A-otMx3 (LOC112247235 Oncorhynchus tshawytscha)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pHAGE-dCas9-3XGFP-CRY2

Plasmid

#249578

Purpose

Lentiviral vector for stable expression of dCas9-3XGFP-CRY2hiclu fusion protein in mammalian cells

Depositor

Tom Misteli

Article

Stortz et al J Cell Sci. 2026 Jan 19:jcs.264574. doi:

Inserts

CRY2hiclu
Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Catalytically dead mutant Cas9

Promoter

CMV

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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Topo_SpCas9.tracrRNA-CS1_AsCas12a DRv10

Plasmid

#237553

Purpose

TOPO vector for the cloning of the SpCas9 tracrRNA-CS1 - AsCas12a Direct Repeat (DR) v10 fragment into the pLCHKO_v4 hgRNA vector for scCHyMErA-Seq experiments.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

SpCas9.tracrRNA-CS1_AsCas12a DRv10

Use

CRISPR

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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iPEmax-WT

Plasmid

#239385

Purpose

For inverse prime editor using Cas9-R221K-N394K in HEK294T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

Cas9-R221K-N393K, M-MLV-RT

Use

CRISPR

Tags

BPNLS and SV40 NLS, c-myc NLS

Expression

Mammalian

Mutation

R221K, N394K in Cas9

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 myc-Rab10-mito

Plasmid

#208367

Purpose

Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10-mito (RAB10 Human)

Expression

Mammalian

Mutation

wildtype

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b+His SUMO Rab10(Q68L)

Plasmid

#207342

Purpose

Bacterial expression of His SUMO Rab10(Q68L)

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

Rab10 (RAB10 Human)

Tags

His-SUMO

Expression

Bacterial

Mutation

Q68L

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-sgHPRT1

Plasmid

#196713

Purpose

CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Insert

Cas9-T2A-BSD-U6-sgHPRT1

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a/hU6

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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GLE_sgRNA

Plasmid

#186658

Purpose

GLE sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Gle sgRNA Plasmid (CG14749 Fly)

Expression

Insect

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Shu_sgRNA

Plasmid

#186656

Purpose

shu sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Shu sgRNA Plasmid (shu Fly)

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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