161,805 results

1. pSpCas9_T2A_mCherry sgBFP

   Plasmid

   #192929

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMVTO-Furin

   Plasmid

   #179920

   Purpose

   Furin protein

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   Furin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBItetDT12nGFP

   Plasmid

   #80420

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 located

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLIX-hN1ICD

   Plasmid

   #91897

   Purpose

   Doxycycline inducible expression of human Notch1 ICD

   Depositor

   Julien Sage

   Insert

   Notch1 intracellular domain (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codons 1770 to 2555 of human NOTCH1

   Promoter

   TRE promoter, Tet ON

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. NAX_CAGGS_CAS9_m4_VPR_EBFP2

   Plasmid

   #167881

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-NSP3SA11

   Plasmid

   #89170

   Purpose

   Express Rotavirus SA11 NSP3 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSGEMKv10.1

   Plasmid

   #85704

   Purpose

   Xenopus laevis oocyte expression of Kv10.1

   Depositor

   Luis Pardo

   Article

   Jenke et al EMBO J. 2003 Feb 3;22(3):395-403.

   Insert

   KCNH1 (KCNH1 Human)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-53-RtcB

   Plasmid

   #51282

   Purpose

   E. coli expression vector for E. coli RtcB RNA ligase

   Depositor

   Jay Hesselberth

   Article

   Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

   Insert

   E. coli RtcB
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNA Polymerase

   Available since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

   Plasmid

   #227246

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α and pTRE3G

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMP71-tCD19-SIINFEKL

    Plasmid

    #174601

    Purpose

    expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitope

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    membrane bound CD19, SIINFEKL (ovalbumin epitope)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-TO-nmdCas9-3XmCherry

    Plasmid

    #64110

    Purpose

    Expression of Nm dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5 alpha6

    Plasmid

    #16036

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    alpha6 (ITGA6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-VP3SA11

    Plasmid

    #89164

    Purpose

    Express Rotavirus SA11 VP3 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promotor

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-HxB.L

    Plasmid

    #65414

    Purpose

    contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

    Depositor

    Harold Erickson

    Article

    Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

    Insert

    TNC large splice variant (TNC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-45b-POLR2A-CTD-mCherry

    Plasmid

    #185011

    Purpose

    Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    POLR2A (Polr2a Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC016 - LwCas13a guide expression backbone with U6 promoter

    Plasmid

    #91906

    Purpose

    Backbone for cloning LwCas13a guides under U6 promoter

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Has Service

    Cloning Grade DNA

    Insert

    LwCas13a Guide site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRL-mU6-GFPGO-IRES-mScarletI

    Plasmid

    #136895

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO-IRES-mScarletI
    

    Use

    Lentiviral

    Tags

    3xNLS on mScarlet

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCVpuro-DEST

    Plasmid

    #119745

    Purpose

    Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Puromycin resistance)

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Benitez-Guijarro et al EMBO J. 2018 Aug 1;37(15). pii: embj.201

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. DRH337: AAV-hsyn-CheRiff-eGFP

    Plasmid

    #51697

    Purpose

    AAV production of CheRiff channelrhodopsin actuator

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    CheRiff-eGFP
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    hSynapsin

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAJE3-E7

    Plasmid

    #214359

    Purpose

    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

    Depositor

    Ryan Mehl

    Article

    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

    Inserts

    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AmpR and lpp

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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