We narrowed to 26,497 results for: Vit;

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p3xFLAG CMV14 / BRAF-V600E

Plasmid

#131723

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

V600E

Promoter

CMV

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mScarlet

Plasmid

#183461

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mScarlet

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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iCAP-BI103-KanR

Plasmid

#203537

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Krienen et al Sci Adv. 2023 Oct 13;9(41):eadk3986. doi

Insert

AAV-BI103 Cap

Use

AAV

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LTB4R2B-DuET

Plasmid

#213334

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

LTB4R2B (LTB4R2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-Puro-dTAG (BRD4)

Plasmid

#91793

Purpose

PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

Puro-P2A-2xHA-FKBP_F36V

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-GenEPi

Plasmid

#140236

Purpose

Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

Depositor

Periklis Pantazis

Article

Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

Insert

GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)

Tags

GCaMP 6s RS1 EF4

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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(attB) tdMCP-tagged BRaf with MS2-circRNA

Plasmid

#226163

Purpose

Used to integrate 3xFlag-tdMCP-BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

3xFlag-tdMCP-BRaf (BRAF Human)
IRES-mCherry-P2A-PuroR
MS2-circRNA barcode

Use

Bxb1 attb, no promoter

Tags

3xFlag-tdMCP

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1622 NOLC1 sgRNA 1

Plasmid

#234833

Purpose

sgRNA 1 for NOLC1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

NOLC1 sgRNA (NOLC1 Human)

Use

CRISPR

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1623 NOLC1 sgRNA 2

Plasmid

#234834

Purpose

sgRNA 2 for NOLC1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

NOLC1 sgRNA (NOLC1 Human)

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

Plasmid

#83842

Purpose

Fluorescent probe for S/G2 cell cycle transition and G2/M transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCRIS-PITChv2-BSD-dTAG (BRD4)

Plasmid

#91792

Purpose

PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

BSD-P2A-2xHA-FKBP_F36V

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promoterless

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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LentiGuide Blast

Plasmid

#199622

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

Depositor

Danwei Huangfu

Article

Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

Plasmid

#204359

Purpose

AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-mCherry-P2A-HA-KORD

Use

AAV

Tags

HA (for KORD) and mCherry (for miniDq)

Mutation

For miniDq, the third intracellular loop (ICL3) o…

Promoter

hSyn

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 WT

Plasmid

#121446

Purpose

expression of huamn PD-L1 wildtype in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A3 WT

Plasmid

#195849

Purpose

Expresses wild-type Homo sapiens ATP13A3

Depositor

Veerle Baekelandt

Article

Houdou et al Biomolecules. 2023 Feb 9;13(2):337. doi:

Insert

ATPase 13A3 (ATP13A3 Human)

Use

Lentiviral

Mutation

D498N, L956P, M850I, V855M, R858H, L675V

Promoter

CMV

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-D614 (C-flag)

Plasmid

#156420

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3

Plasmid

#75127

Purpose

Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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SpCas9-HF1-plus

Plasmid

#126768

Purpose

Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1-plus

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A; Q695A; Q926A; amino acids 1005-1013 replac…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-DI-2A

Plasmid

#74671

Purpose

Mammalian expression of DI-2A (hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Article

De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

Inserts

CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Tags

F2A

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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CXCR5-DuET

Plasmid

#213222

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CXCR5 (CXCR5 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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