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pJZC572

Plasmid

#62318

Purpose

sgRNA with 1x com for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x com RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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Man1B1-Myc

Plasmid

#163645

Purpose

Expresses myc tagged Man1B1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mannosidase alpha class 1B member 1 (MAN1B1 Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GST-rhodopsin-CTS

Plasmid

#86071

Purpose

A bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Rhodopsin (RHO Bovine)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 309-348 of Rhodopsin

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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LB595 (Bcl-2 promoter CRE mut)

Plasmid

#15243

Depositor

Linda Boxer

Article

Heckman et al Oncogene. 2002 May 30. 21(24):3898-908.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Mutated CRE site at -1537 changed from GTGACGTTA …

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
D44_Ld_180090

Plasmid

#135858

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

alpha glucosidase II subunit, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLib6.6B

Plasmid

#176666

Purpose

Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXL2-coemiRFP670-PGK-Puro

Plasmid

#186168

Purpose

Knock-in gene targeting in marmoset cells at FOXL2 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

emiRFP670

Use

Marmoset targeting

Mutation

human codon-optimized

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP6-A66S_rpoA-U

Plasmid

#190989

Purpose

Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:A66S

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP2-A66S_rpoA-U

Plasmid

#190977

Purpose

Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Mammalian

Mutation

DYW-KP:A66S

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfKitlgb

Plasmid

#168200

Purpose

Express zebrafish Kitlgb, mRNA synthesis

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlgb (kitlgb Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Mutation

I85V, V222I, and D233N- please see depositor comm…

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFX-GalT-SECFP

Plasmid

#174470

Purpose

Express SECFP with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with golgi apparatus-targeting sequence

Tags

β1,4-galactose transferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-SECFP

Plasmid

#174185

Purpose

Express SECFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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p3A9cbetaTCR.20beta

Plasmid

#69592

Purpose

TCR beta chain genomic transgenic construct for creating OT-II mice

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Insert

TCR beta VJ region from clone 1.1 (Tcrb Mouse)

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pCsy-crRNA-EV

Plasmid

#153944

Purpose

For cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-MKRN1 H307E

Plasmid

#78749

Purpose

To overexpress MKRN1 H307E in Mammalian Cell

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

HA

Expression

Mammalian

Mutation

H307E

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pFX-GalT-iRFP

Plasmid

#174474

Purpose

Express iRFP with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with golgi apparatus-targeting sequence

Tags

β1,4-galactose transferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-H2B/pcDNA3

Plasmid

#51971

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

human H2B

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern-Actin/pEGFP-C1

Plasmid

#51972

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

Actin

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pLJC2-UMPS (Y37A, R155A)-3xFLAG

Plasmid

#87976

Purpose

Expression of UMPS (Y37A, R155A) in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

UMPS (Y37A, R155A) (UMPS Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Y37A, R155A

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC2-Rap2A-3xFLAG

Plasmid

#87974

Purpose

3rd generation lentiviral vector for Expression of Rap2A in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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