159,557 results

1. EB3N-VVDfast-mCl3-iLID

   Plasmid

   #190165

   Purpose

   Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   EB3N-VVDfast-mClover3-iLID
   

   Use

   Tags

   -VVDfast-mClover3-iLID

   Expression

   Mammalian

   Mutation

   1-189 aa from EB3

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Reporter-2

   Plasmid

   #180219

   Purpose

   Surrogate reporter plasmid for the assay of targeted RNA editing

   Depositor

   Wensheng Wei

   Article

   Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

   Insert

   Surrogate reporter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ND4-DdCBE- left side TALE

   Plasmid

   #157844

   Purpose

   expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pC0056 - LwCas13a-msfGFP-NES

   Plasmid

   #105815

   Purpose

   Expresses active LwaCas13a-NES for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR and Lentiviral

   Tags

   NES and msfGFP-NES-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAB076i3

   Plasmid

   #79198

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-sadCas9-VP64

   Plasmid

   #115790

   Purpose

   An AAV vector expressing S. aureus dCas9 fused to VP64.

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   Sa-dCas9
   

   Use

   AAV

   Tags

   NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C1-MPAct-mCitrine

   Plasmid

   #155220

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFH2.22_mCherry-MS2x8

   Plasmid

   #205537

   Purpose

   Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNat-AID*-9myc

   Plasmid

   #99523

   Purpose

   C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-116) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid

    #225684

    Purpose

    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

    Depositor

    Ryan Mehl

    Inserts

    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    

    Use

    Methanomethylophilus alvus

    Tags

    Expression

    Mutation

    CTG125CTT, N166A, V168S, A223C, W239R

    Promoter

    GlnS and Lpp

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge