159,557 results

1. pGES201

   Plasmid

   #190197

   Purpose

   For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

   Depositor

   Yuefeng Guan

   Article

   Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   plant-codon optimized

   Promoter

   Glycine max elongation factor 1A(pM4)

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. APEX2-OMM

   Plasmid

   #79056

   Purpose

   Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Soybean APEX2
   

   Use

   Lentiviral

   Tags

   flag and last 31 amino acids of human MAVS

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV immearly promotor

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG425GPD-ccdB

   Plasmid

   #14154

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pShHELIX_sgRNA_entry (CJT32)

   Plasmid

   #181782

   Purpose

   Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti CMV GFP Hygro (656-4)

   Plasmid

   #17446

   Purpose

   3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Lentiviral Prep

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

   Plasmid

   #129631

   Purpose

   Visualization of Akt and ERK activity

   Depositor

   Dorus Gadella

   Article

   Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

   Insert

   H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLSB-KAN

   Plasmid

   #166700

   Purpose

   Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   adh15 promoter and tRNA Ser promoter

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJJB302

   Plasmid

   #107694

   Purpose

   OsActin Promoter-LwaCas13a-HSP Terminator

   Depositor

   Daniel Voytas

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   Cas13a
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsActin

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEmerald-Rad21-N-18

   Plasmid

   #54248

   Purpose

   Localization: Centromere, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Rad21 (RAD21 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. AAVS1_Puro_hPGK1_EGFP_Donor

    Plasmid

    #178088

    Purpose

    HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge