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pJM453 EF1alpha EBFP2-P2A-BlastR (TUPV4)

Plasmid

#139233

Purpose

mMoClo TUPV4, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM452 EF1alpha EBFP2-P2A-BlastR (TUPV3)

Plasmid

#139232

Purpose

mMoClo TUPV3, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJM451 EF1alpha EBFP2-P2A-BlastR (TUPV2)

Plasmid

#139231

Purpose

mMoClo TUPV2, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

Plasmid

#160609

Purpose

TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:MS2-VP64:Tnos

Use

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

Plasmid

#160625

Purpose

GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

U6-26-1gRNA-DFR 2.1

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

U6-26

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3

Plasmid

#128764

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #3 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

Plasmid

#128763

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #2 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

Plasmid

#128762

Purpose

Expresses DIS3L2 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

DIS3L2 sgRNA #1 (DIS3L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

Plasmid

#128760

Purpose

Expresses PARN gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PARN sgRNA #2 (PARN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

Plasmid

#128758

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #3 (TENT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

Plasmid

#128756

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

Plasmid

#128757

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZ-pre-Ost1-pro-alphaf-Btl2

Plasmid

#117665

Purpose

Study secretion efficiency of Btl2

Depositor

Benjamin Glick, Pau Ferrer

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-pro-alphaf-Btl2

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the lipase Btl2 together wit…

Promoter

pAOX1

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

Plasmid

#117663

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Benjamin Glick, Pau Ferrer

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pOst1-pro-af(MUT2)-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala

Plasmid

#179716

Purpose

Lentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

Plasmid

#118593

Purpose

Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha/TRE3G

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

Plasmid

#141385

Purpose

Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-E (E SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

Plasmid

#141386

Purpose

Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-M (M SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

Plasmid

#141391

Purpose

Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-N (N SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
NYESO alpha into TRAC HDRT Source (pTR 223)

Plasmid

#112023

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge