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FRP2375

Plasmid

#127581

Purpose

Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and NAT resistance gene

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PtetO7.1_Flagtag_8Glycine linker

Use

Synthetic Biology; Pcr based tagging

Tags

Flag tag, 8glycine linker

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMKV058

Plasmid

#133313

Purpose

BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc+. WUS2

Expression

Plant

Promoter

AtUbi10, Nos

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLew100::NLS-ISceI-HA

Plasmid

#21299

Depositor

Nina Papavasiliou

Article

Boothroyd et al Nature. 2009 May 14;459(7244):278-81. do

Insert

I-SceI (SCEI )

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pBS-iNOS

Plasmid

#19295

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1992 Aug 1. 89

Insert

inducible nitric oxide synthase (Nos2 Mouse)

Expression

Bacterial

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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FRP2374

Plasmid

#127579

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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FRP2365

Plasmid

#127575

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApaLID211A-HA

Plasmid

#211459

Purpose

Express mitochondrial localized ApaLI with D211A mutation

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-D211A

Tags

HA

Expression

Mammalian

Mutation

D211A

Promoter

CMV

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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bRA66

Plasmid

#100952

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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nSYB-GAL80-DD

Plasmid

#124778

Purpose

Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

Depositor

Jing-W Wang

Article

Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

Insert

TMP-inducible destabilized GAL80 (GAL80-DD)

Tags

DD

Expression

Insect

Promoter

nSynaptobrevin

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3_FLAG_MTA3

Plasmid

#31696

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Metastasis associated protein 3 (MTA3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT

Plasmid

#19291

Depositor

Eric Fearon

Article

Hajra et al Cancer Res. 2002 Mar 15. 62(6):1613-8.

Insert

E-cadherin

Use

Luciferase

Expression

Mammalian

Mutation

E-cad promoter sequences from -108 to +125 Ebo…

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
bRA77

Plasmid

#100953

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-PAX6a

Plasmid

#222576

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of PAX6a

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

PAX6a (PAX6 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFPGW-UAS-Citrine

Plasmid

#125232

Purpose

For generating UAS-Citrine lentivirus

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

UAS-Citrine

Use

Lentiviral

Expression

Mammalian

Promoter

UAS

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC.HA Vav3

Plasmid

#14555

Depositor

Joan Brugge

Article

Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

Insert

Vav3 (Vav3 Mouse)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pEQ1180

Plasmid

#83805

Purpose

express human cytomegalovirus TRS1 with a C-terminal 6X his tag in eukaryotic cells

Depositor

Adam Geballe

Article

Child et al J Virol. 2012 Apr;86(7):3880-9. doi: 10.

Insert

TRS1

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
FRP2372

Plasmid

#127578

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JP1520 PIK3CA WT HA

Plasmid

#14570

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA (PIK3CA )

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GFP-SynaptophysW

Plasmid

#27235

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

Synaptophys-GFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pZZMP122

Plasmid

#231756

Purpose

Mammalian expression of SPO11-YF

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-YF (Spo11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Y138F

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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