We narrowed to 2,961 results for: NAK

1. peSpCas9(1.1)-2×sgRNA (empty, donor)

   Plasmid

   #80768

   Purpose

   All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pG2J

   Plasmid

   #102882

   Purpose

   Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Inserts

   OsTIR1
   mGFP-AID
   

   Use

   Tags

   AID(71-114)

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 and TEF1

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Myc-CEBPA

   Plasmid

   #219393

   Purpose

   Express CEBPA in mammalian cells

   Depositor

   Yoichi Furukawa

   Article

   Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

   Insert

   CEBPA (CEBPA Human)
   

   Use

   Tags

   N terminal Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 1099 pcDNA GFP FKHR AAA

   Plasmid

   #9023

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   FKHR AAA (FOXO1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T24A S256A S319A

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-CHAMP1

   Plasmid

   #159892

   Purpose

   Expresses CHAMP1 (CAMP) in mammalian cells

   Depositor

   Kozo Tanaka

   Article

   Itoh et al EMBO J. 2011 Jan 5;30(1):130-44. doi: 10

   Insert

   CHAMP1 (CHAMP1 Human)
   

   Use

   Tags

   pEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

   Plasmid

   #173871

   Purpose

   Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1-IFT43

   Plasmid

   #218735

   Purpose

   Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

   Insert

   IFT43 (IFT43 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Myc-FOXA1

   Plasmid

   #219394

   Purpose

   Express FOXA1 in mammalian cells

   Depositor

   Yoichi Furukawa

   Article

   Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

   Insert

   FOXA1 (FOXA1 Human)
   

   Use

   Tags

   N terminal Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-EGFP-C-IFT81

   Plasmid

   #218732

   Purpose

   Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

   Insert

   IFT81 (IFT81 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNAz-mBACEflag (C474A/C478A/C482A/C485A)

    Plasmid

    #26736

    Depositor

    Gopal Thinakaran

    Article

    Vetrivel et al J Biol Chem. 2009 Feb 6. 284(6):3793-803

    Insert

    BACE (Bace1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cys 474, 478, 482, and 485 mutated to Ala

    Promoter

    Available since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-HA-GBF1(E794K)

    Plasmid

    #206416

    Purpose

    Mammalian expression of hGBF1(E794K)

    Depositor

    Kazuhisa Nakayama

    Article

    Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

    Insert

    GBF1 (GBF1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E794K

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. dmBACCS2NS-IRES-dOrai-IRES-GFP

    Plasmid

    #72895

    Purpose

    Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2NS-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. dmBACCS2-IRES-dOrai-IRES-GFP

    Plasmid

    #72893

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-C1-IFT27

    Plasmid

    #218722

    Purpose

    Expresses N-terminally EGFP-tagged IFT27 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT27 (IFT27 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Igfbp1-promoter/pGL3

    Plasmid

    #12146

    Depositor

    Domenico Accili

    Article

    Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

    Insert

    IGFBP1 promoter (Igfbp1 Rat)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains the canonical insulin response sequence …

    Promoter

    Available since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA4-Xpress-GBF1

    Plasmid

    #206417

    Purpose

    Mammalian expression of hGBF1

    Depositor

    Kazuhisa Nakayama

    Article

    Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

    Insert

    GBF1 (GBF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1037 pGL3 FasL promoter

    Plasmid

    #9028

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    Fas ligand promoter (FASLG Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

19. 1129 pcDNA3 GFP FKHR H215R AAA

    Plasmid

    #9034

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R AAA (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H215R T24A S256A S319A

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

20. mCherry-PHR-iSH-T2A-CIBNcaax

    Plasmid

    #173860

    Purpose

    Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)

    Depositor

    Takao Nakata

    Article

    Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

    Insert

    mCherry-PHR-iSH-T2A-CIBNcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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