Addgene

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HA-Foxo1ADA (pCMV5)

Plasmid

#12143

Depositor

Domenico Accili

Article

Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

Insert

Foxo1 ADA (Foxo1 Mouse)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Phosphorylation-deficient, also referred to as co…

Promoter

CMV

Available since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

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Myc-Foxo1 D256

Plasmid

#12145

Depositor

Domenico Accili

Article

Nakae et al EMBO J. 2000 Mar 1. 19(5):989-96.

Insert

Foxo1 (Foxo1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Contains a deletion truncating the protein after …

Promoter

CMV

Available since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA C12 ecDHFR DD YFP HA

Plasmid

#192815

Purpose

Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

Depositor

John Hulleman

Article

Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

Insert

E. coli dihydrofolate reductase

Use

Tags

enhanced yellow fluorescent protein and hemagglut…

Expression

Mammalian

Mutation

W74R/T113S/E120D/Q146L

Promoter

CMV

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT88

Plasmid

#218733

Purpose

Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

Insert

IFT88 (IFT88 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-CEBPA

Plasmid

#219393

Purpose

Express CEBPA in mammalian cells

Depositor

Yoichi Furukawa

Article

Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

Insert

CEBPA (CEBPA Human)

Use

Tags

N terminal Myc tag

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT43

Plasmid

#218735

Purpose

Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

IFT43 (IFT43 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT81

Plasmid

#218732

Purpose

Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

Insert

IFT81 (IFT81 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-CHAMP1

Plasmid

#159892

Purpose

Expresses CHAMP1 (CAMP) in mammalian cells

Depositor

Kozo Tanaka

Article

Itoh et al EMBO J. 2011 Jan 5;30(1):130-44. doi: 10

Insert

CHAMP1 (CHAMP1 Human)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-FOXA1

Plasmid

#219394

Purpose

Express FOXA1 in mammalian cells

Depositor

Yoichi Furukawa

Article

Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Tags

N terminal Myc tag

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT74

Plasmid

#218730

Purpose

Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

Insert

IFT74 (IFT74 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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peSpCas9(1.1)-2×sgRNA (empty, donor)

Plasmid

#80768

Purpose

All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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dmBACCS2-IRES-dOrai-IRES-mCherry

Plasmid

#72894

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai-IRES-mCherry

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-beta-catenin-S33Y

Plasmid

#13371

Purpose

Retroviral expression of mouse beta-catenin S33Y mutant

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

catenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Serine 33 to Tyrosine and Leucine 388 to Proline

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCXLE-hGLIS1

Plasmid

#37625

Purpose

Non-integrating (episomal) expression of human GLIS1

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

human GLIS1 (GLIS1 Human)

Use

Tags

Expression

Mammalian

Mutation

T110A, A187G, I221T

Promoter

CAG

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Plasmid

#173871

Purpose

Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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1099 pcDNA GFP FKHR AAA

Plasmid

#9023

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR AAA (FOXO1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

T24A S256A S319A

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNAz-mBACEflag (C474A/C478A/C482A/C485A)

Plasmid

#26736

Depositor

Gopal Thinakaran

Article

Vetrivel et al J Biol Chem. 2009 Feb 6. 284(6):3793-803

Insert

BACE (Bace1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Cys 474, 478, 482, and 485 mutated to Ala

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-GBF1(E794K)

Plasmid

#206416

Purpose

Mammalian expression of hGBF1(E794K)

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E794K

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pG2J

Plasmid

#102882

Purpose

Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Inserts

OsTIR1
mGFP-AID

Use

Tags

AID(71-114)

Expression

Yeast

Mutation

Promoter

ADH1 and TEF1

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-NUP188

Plasmid

#159893

Purpose

Expresses NUP188 in mammalian cells

Depositor

Kozo Tanaka

Article

Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

Insert

NUP188 (NUP188 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

To avoid NotI site, first three amino acids are d…

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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