We narrowed to 13,830 results for: RING;

1. pLD-puro-Cc-CR-PARK7WT-VA

   Plasmid

   #115187

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLD-puro-Cc-CR-PARK7V51G-VA

   Plasmid

   #115188

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLD-puro-Cc-CR-PARK7C53A-VA

   Plasmid

   #115189

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLD-puro-Cc-CR-PARK7H126A-VA

   Plasmid

   #115190

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLD-puro-Cc-CR-PARK7E163K-VA

   Plasmid

   #115191

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLEX307_CR-PDHA2

   Plasmid

   #115199

   Purpose

   Lentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLEX307_CR-PDHA2S291A

   Plasmid

   #115203

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HS_NAM-B1

   Plasmid

   #154064

   Purpose

   Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Inserts

   Hygromycin phosphotransferase
   Cre recombinase
   LoxP-flanked GUS and TtNAM-B1
   

   Use

   Cre/Lox and Synthetic Biology; Golden gate

   Tags

   Expression

   Bacterial and Plant

   Mutation

   The TtNAM-B1 gene sequence was domesticated to re…

   Promoter

   HvHSP17, OsAct, and ZmUbi

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP-SFFV-tagBFP-2A-ICP0-FxE

   Plasmid

   #235539

   Purpose

   To express tagBFP-2A-ICP0-FXE (ICP0 ∆106-149)

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

   Insert

   ICP0-FxE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of the RING domain, ∆106-149.

   Promoter

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn T21R-F8.2 (S54L+T127A)-T2A-mCherry

    Plasmid

    #225590

    Purpose

    AAV transgene plasmid with hSyn promoter for expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-F8.2 (S54L+T127A)-T2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-4T-3-TRC8 513-664

    Plasmid

    #185352

    Purpose

    RING domain-containing GST fusion protein for ubiquitination assays.

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    TRC8 (RNF139 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 513-664 only

    Promoter

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

    Plasmid

    #225588

    Purpose

    Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-F8.2(S54L+T127A)-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry

    Plasmid

    #225584

    Purpose

    Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-F8.2 (S54L+T127A)-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T21R-F8.2 (S54L+T127A)

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMAL-PIAL2M-sim1

    Plasmid

    #67908

    Purpose

    Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

    Depositor

    Andreas Bachmair

    Article

    Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

    Insert

    PIAL2 (AT5G41580 Mustard Weed)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    E281-A496; VEDL 425-428 AAA

    Promoter

    P-lac

    Available Since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCMV5D HA-PPM1H_M flap

    Plasmid

    #236718

    Purpose

    Expresses PPM1H with PPM1M flap domain sequence

    Depositor

    Suzanne Pfeffer

    Article

    Claire Y. et al bioRxiv, 2025

    Insert

    PPM1H_M flap (PPM1H, PPM1M Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PPM1H flap domain replaced with PPM1M flap domain

    Promoter

    CMV

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV5D HA-PPM1M_H flap

    Plasmid

    #236719

    Purpose

    Expresses PPM1M with PPM1H flap domain sequence

    Depositor

    Suzanne Pfeffer

    Article

    Claire Y. et al bioRxiv, 2025

    Insert

    PPM1M_H flap (PPM1M, PPM1H Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PPM1M flap domain replaced with PPM1H flap domain

    Promoter

    CMV

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRosettav2

    Plasmid

    #115557

    Purpose

    To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments

    Depositor

    John Doench

    Insert

    Hygromycin-Blasticidin-Puromycin-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSAH031

    Plasmid

    #90330

    Purpose

    Promoterless iLOV gene for recombineering, flanked by BsmbI restriction sites.

    Depositor

    David Savage

    Insert

    iLOV
    

    Use

    Tags

    None

    Expression

    Mutation

    Promoter

    None

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ATSR

    Plasmid

    #50052

    Purpose

    expresses t.gondii VWA+TSR1 domains in mammalian cells

    Depositor

    Timothy Springer

    Insert

    MIC2-ATSR
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    S158A

    Promoter

    Chick beta-actin promoter

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. proATSR

    Plasmid

    #50053

    Purpose

    Expresses T.gondii prodomain+VWA+TSR1 domains in mammalian cells

    Depositor

    Timothy Springer

    Insert

    MIC2-proATSR
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    S158A

    Promoter

    chick beta-actin promoter

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only