We narrowed to 17,086 results for: URE

1. pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

   Plasmid

   #138295

   Purpose

   gRNA for CRISPR/Cas9 knockout of human TRIM21

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   gRNA TRIM21 (TRIM21 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TDP-43_CTD_A321V

   Plasmid

   #98671

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321V

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A321V

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti_CamKIIa_mKate2

   Plasmid

   #96942

   Purpose

   To label mature excitatory iPSC-derived human neurons

   Depositor

   Ricardo Dolmetsch, Oleksandr Shcheglovitov

   Article

   Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

   Insert

   far-red fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   E87D (please see depositor comments below)

   Promoter

   CaMKIIa

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-OC-IRES-BSD

   Plasmid

   #53118

   Purpose

   To create stable cell clones with high-level expression of Cas9 and OCT1

   Depositor

   Wensheng Wei

   Article

   Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Inserts

   OCT1 (POU2F1 Human)
   Cas9
   

   Use

   Lentiviral

   Tags

   IRES-BSD, NLS, and P2A

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

5. QUAS-jGCaMP7s-T2A-tdTomato

   Plasmid

   #200010

   Purpose

   QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

   Depositor

   Carolyn McBride

   Article

   Zhao et al Nature. 2022 May;605(7911):706-712. doi:

   Insert

   3xP3-ECFP-jGCaMP7s-td-Tomato
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

   Plasmid

   #47457

   Purpose

   LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   NLS(alpha-imp)-CRY2PHR-NLS-VP64
   

   Use

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TDP-43_CTD_A321G

   Plasmid

   #98670

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321G

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A321G

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRK5-FLAG-C12orf66

   Plasmid

   #87047

   Purpose

   mammalian expression of C12orf66

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   C12orf66 (C12orf66 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK5-FLAG-ITFG2

   Plasmid

   #87044

   Purpose

   mammalian expression of ITFG2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   ITFG2 (ITFG2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TDP-43_CTD_M337P

    Plasmid

    #98675

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337P

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M337P

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TDP-43_CTD_A326P

    Plasmid

    #98672

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with A326P

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A326P

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV10-3xFlag Skp2 delta-F

    Plasmid

    #81116

    Purpose

    mammalian expression of F-box deleted Skp2

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    SKP2 (SKP2 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    deleted F box

    Promoter

    CMV

    Available since

    Sept. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pRSV-CaMKIV

    Plasmid

    #45062

    Purpose

    Expression of CaMKIV

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    Cam Kinase IV (Camk4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    *

    Promoter

    RSV

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1P shFXN_1

    Plasmid

    #102970

    Purpose

    Suppression of FXN

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shFXN_1 (FXN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1P shFXN_2

    Plasmid

    #102971

    Purpose

    Suppression of FXN

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shFXN_2 (FXN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKS18

    Plasmid

    #24629

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    mFar1 (Far1 Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. plc242-GFP-ITFG2

    Plasmid

    #87049

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-MYC-ITFG2

    Plasmid

    #87046

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47454

    Purpose

    epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    CRY2PHR-NLS-SID4X
    

    Use

    AAV

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge