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HA-cNM2A-Flag
Plasmid#236532PurposeFor expression of NM2A (dog) - with HA and FLAG tags in mammalian cellsDepositorInsertMYH9
UseTagsFLAG and HAExpressionMammalianMutationWTPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pYI2206
Plasmid#228348PurposeConstitutive rPhlTA mutant expressionDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1026
Plasmid#228299PurposeConstitutive expression of PhlTA mutant with weak promoter and terminatorDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationE41GPromoterKpYPT1 promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT478
Plasmid#228286PurposeConstitutive mutant rPhlTA expression with strong promoterDepositorInsertrphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Monbr-Sox-like
Plasmid#231530PurposeExpresses Monsiga brevicolis full-length Sox-like in mammalian cells, for lentivirus generationDepositorInsertSox-like
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Chim-Salhel-I-K57E
Plasmid#231519PurposeExpresses Salpingoeca helianthica chimeric Sox-I mutant in mammalian cells, for lentivirus generationDepositorInsertSalhel-I HMG K57E flanked with N- and C-termini of mSox2
UseLentiviralTagsExpressionMutationPoint mutation at position 57 K to E of the HMGPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Salro-Sox-like
Plasmid#231531PurposeExpresses Salpingoeca rosetta full-length Sox-like in mammalian cells, for lentivirus generationDepositorInsertSox-like
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
tdiRFP-BFP reporter
Plasmid#190184PurposeThe cre-activatable fluorescence reporterDepositorInsertstdiRFP
TagBFP2
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-HIS3
Plasmid#232093PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRTagsExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Hyg-ADE2
Plasmid#232104PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Kan-ADE2
Plasmid#232105PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-Nat
Plasmid#232090PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRTagsExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7CNW)RS
Plasmid#222874PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-Kan
Plasmid#232092PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRTagsExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(5CNW)RS
Plasmid#222872PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-NS3/4A S139A
Plasmid#186885PurposeDoxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transductionDepositorInsertHCV NS3/4A protease with S139A mutation
UseRetroviralTagsExpressionMutationS139APromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-WHG cGAS-HA
Plasmid#186867PurposeDoxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transductionDepositorInsertWhite-handed gibbon cGAS with C-terminal HA tag
UseRetroviralTagsHAExpressionMutationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP8B3-HA
Plasmid#209211PurposeMammalian expression of ATP8B3DepositorInsertATP8B3 (ATP8B3 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only
