We narrowed to 10,632 results for: t7

1. pET28a-HsSENP3

   Plasmid

   #71467

   Purpose

   Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Flag-Kdm6b(1025-End)-pA

   Plasmid

   #100278

   Purpose

   In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

   Insert

   Kdm6b (Kdm6b Mouse)
   

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Mutation

   catalytic domain (1025aa-End)

   Promoter

   T7

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH610

   Plasmid

   #131862

   Purpose

   Produces holo-PhyB* in E. coli

   Depositor

   Wilfried Weber

   Article

   Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

   Inserts

   PhyB(1-651)-RGD-His6-Cys
   HO1
   PcyA
   

   Expression

   Bacterial

   Promoter

   T7 promoter-1 and T7 promoter-2

   Available Since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL1 eforRed

   Plasmid

   #106320

   Purpose

   Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   eforRed
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. DT044A

   Plasmid

   #159405

   Purpose

   Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7/LacO promoter

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-NtC60αESNtLC60β

   Plasmid

   #160878

   Purpose

   Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcL, cpn60α, cpn60β, groES
   

   Expression

   Bacterial

   Promoter

   rbcL: T7, chaperones: T7

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6x-His_A1aY1_GFP

   Plasmid

   #158753

   Purpose

   Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

   Insert

   6xHistidine_A1aY1_GFP
   

   Tags

   6x-Histidine tag, GFP, T7 leader sequence, and Th…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETcLIC-GFP

   Plasmid

   #183514

   Purpose

   The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

   Depositor

   Martin Högbom

   Article

   Grave et al Protein Expr Purif. 2022 Oct;198:106132.

   Type

   Empty backbone

   Tags

   10x His-tag and folding reporter GFP

   Expression

   Bacterial

   Promoter

   T7 lac

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL0915 (CRISPR with target 3)

   Plasmid

   #130643

   Purpose

   Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   CRISPR(target-3)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-2H12

    Plasmid

    #221186

    Purpose

    His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRBC-sfGFP-134/150TAG

    Plasmid

    #174077

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    D134TAG + N150TAG

    Promoter

    T7

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. T7RNAP Cyslight V174C

    Plasmid

    #205078

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S V174C

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUDE1084

    Plasmid

    #168500

    Purpose

    expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

    Depositor

    Jean-Marc Daran

    Article

    Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

    Inserts

    Fncas12a
    T7RNA polymerase
    

    Use

    CRISPR

    Promoter

    TDH3 and TEF1

    Available Since

    Dec. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET23a-HsUBB

    Plasmid

    #69562

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (UBB Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pSL0426 (CRISPR with spc3)

    Plasmid

    #130642

    Purpose

    Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    CRISPR(spacer-3)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-C4-mCherry

    Plasmid

    #66530

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter C4

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - C4

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b I-SceI (Ctermhis -MH)

    Plasmid

    #195613

    Purpose

    Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-NtC60αC20NtLC60β

    Plasmid

    #160877

    Purpose

    Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcL, cpn60α, cpn60β, cpn20
    

    Expression

    Bacterial

    Promoter

    rbcL: T7, chaperones: T7

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEU3-NII-GLICNot-C-His

    Plasmid

    #140187

    Purpose

    Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site

    Depositor

    Tamás Mészáros

    Article

    Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

    Type

    Empty backbone

    Use

    Expression vector for a cell-free system

    Tags

    6xHis and GST

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-agrBD-I

    Plasmid

    #53439

    Purpose

    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    agrBD locus
    

    Use

    Synthetic Biology

    Tags

    V5 epitope, 6x HIS (not expressed on agrB or agrD…

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7-lac

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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