We narrowed to 30,867 results for: PLE;

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pJOG1368

Plasmid

#167301

Purpose

Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

FCY-UPP fusion gene

Use

Synthetic Biology; Moclo level 0 vector

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A/T1462A)

Plasmid

#14131

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A/T1462A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A/T1462A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
gRNA-ura-HYB

Plasmid

#64330

Purpose

ura3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of ura3 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCARGO-RMCE-Xist

Plasmid

#233268

Purpose

CARGO plasmid containing ~22kb dox-inducible Xist transgene

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

22kb Xist gene, including introns

Expression

Mammalian

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AktAR

Plasmid

#118462

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSLC-254

Plasmid

#124291

Purpose

Expression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.

Depositor

Swaine Chen

Article

Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

Insert

vsfGFP-0

Expression

Bacterial

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTP2620

Plasmid

#113311

Purpose

human Rad50 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Rad50 (RAD50 Human)

Tags

HA, 6xhis

Expression

Insect

Promoter

polyhedrin

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCX-EGFP beta5 integrin receptor

Plasmid

#14996

Depositor

Raymond Birge

Article

Albert et al Nat Cell Biol. 2000 Dec . 2(12):899-905.

Insert

beta5 integrin receptor (ITGB5 Human)

Tags

IRES EGFP

Expression

Mammalian

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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paoB-CBE

Plasmid

#220373

Purpose

for hA3A-nCas9 expression in Aspergillus oryzae

Depositor

Xue Gao

Article

Zhao et al J Am Chem Soc. 2023 Jan 11;145(1):413-42

Insert

hA3A-nCas9

Use

CRISPR

Promoter

Ptef1

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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EYFP Peroxisome visual barcode

Plasmid

#158677

Purpose

tagging clonal populations with Peroxisomal EYFP fluorescent protein. Adding Peroxisome localization signal to EYFP WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

EYFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTet-β-tubulin_SNAP

Plasmid

#136831

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1749 (pDonor, R*MmeI)

Plasmid

#160733

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA

Expression

Bacterial

Promoter

N/A

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRAN473

Plasmid

#120813

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

Insert

red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pME18s-HA-hFIP200

Plasmid

#24303

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

FIP200 (RB1CC1 Human)

Tags

3xHA

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6

Plasmid

#197967

Purpose

Mammalian cell expression of wt 3xFLAG-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pActin-miRFP670nano3

Plasmid

#184665

Purpose

Human beta-actin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-human beta-actin

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-DEST

Plasmid

#106348

Purpose

Destination vector for gateway cloning. Developed for Flp-In T-REx system.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Gateway entry vector

Expression

Mammalian

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 bovine GRK2

Plasmid

#14691

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 (GRK2 Bovine)

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
myc EDC4 pCMVSport6

Plasmid

#86855

Purpose

mammalian expression of EDC4

Depositor

Sarah Slavoff

Article

D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

Insert

EDC4 (EDC4 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Ls-AChBP

Plasmid

#63126

Purpose

Expression of Lymnaea stagnalis AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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