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pDGE660

Plasmid

#153258

Purpose

M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE661

Plasmid

#153259

Purpose

M5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2042.2_Bs

Plasmid

#140671

Purpose

1-color stripe (“BLUE”)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

1-color stripe (“BLUE”)

Use

Synthetic Biology

Expression

Bacterial

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-P2rP4

Plasmid

#141015

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME-nEGFP

Plasmid

#138363

Purpose

Gateway compatible middle entry clone contains nuclear EGFP sequences.

Depositor

Nathan Lawson

Publication

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

nEGFP

Expression

Mammalian

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAW203

Plasmid

#113243

Purpose

E. coli expression vector for IHFa E10A + IHFb

Depositor

Jennifer Doudna

Publication

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa E10A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

E10A

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAM4645

Plasmid

#85124

Purpose

NSII vector expressing PkaiB-KaiBC. Nt resistant. Complements rhthyms in AMC705

Depositor

Susan Golden

Publication

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

PkaiBC-KaiBC

Expression

Bacterial

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pZS2katGp-RBS31-PhiC31-proD-oxyR

Plasmid

#78213

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pNX3d-HA3

Plasmid

#78380

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
ura4+ (ura4 Fission Yeast)

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3a-FL3

Plasmid

#78358

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
kanMX6

Expression

Yeast

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD20-GFP

Plasmid

#80651

Purpose

Promoter1 with BCD20-gfp

Depositor

Nathan Hillson

Publication

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD21-GFP

Plasmid

#80652

Purpose

Promoter1 with BCD21-gfp

Depositor

Nathan Hillson

Publication

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pMM750

Plasmid

#68899

Purpose

IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Publication

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMM732

Plasmid

#68897

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Publication

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHL1632

Plasmid

#62829

Purpose

Lim Lab plasmid: AmpR + PLlacO-1::RBS(st7) gfp::Asp terminator + ColE1

Depositor

Han Lim

Publication

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Insert

gfp

Use

Synthetic Biology

Tags

st7 RBS

Expression

Bacterial

Promoter

pLlacO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
mEGFP-EF(N)-30nmSAH

Plasmid

#61679

Purpose

GFP-labeled SAH scaffold with EF Hand attachment site

Depositor

Kristen Verhey

Publication

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-EF(N)-30nmSAH

Expression

Mammalian

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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LI D-E 6xHIS

Plasmid

#54447

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

polyhistidine (6x) tag

Use

Cloning vector

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D Cerulean c1

Plasmid

#54417

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CFP Cerulean

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI A-B Ubi (+ intron) c4

Plasmid

#54408

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

L. japonicus polyubiquitin promoter

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRGM10

Plasmid

#48790

Purpose

I-SceI DSB repair reporter cassette

Depositor

Michael Glickman

Publication

Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

Inserts

lacZ(I-SceI)
lacZ-deltaN

Use

Dsb repair reporter

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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