179,359 results

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pESF140_HisSpy_DDB1∆B

Plasmid

#124213

Purpose

Insect cell expression vector for His6, Spy tagged hsDDB1∆B

Depositor

Eric Fischer

Publication

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

DNA damage-binding protein 1 (DDB1 Human)

Tags

His6-Spy

Expression

Insect

Mutation

internal deletion of BPB domain to aid crystalliz…

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Publication

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Expression

Mammalian

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHAGE-CDK4

Plasmid

#116724

Purpose

Lentiviral expression of CDK4

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-6p1-GST-ArcFL

Plasmid

#119877

Purpose

Bacterial Expression construct for protein production and purification

Depositor

Jason Shepherd

Publication

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Expression

Bacterial

Promoter

tac

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMK346 (Hygro-P2A-mAID-mCherry)

Plasmid

#121180

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Hygro-P2A-mAID-mCherry

Use

CRISPR

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMaCTag-P05

Plasmid

#120016

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

Plasmid

#117728

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Expression

Bacterial

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag-GFP11-pDEST-N

Plasmid

#118366

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-Flag

Expression

Mammalian

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CMV Flag ATRwt

Plasmid

#41909

Depositor

Stephen Elledge

Publication

Cortez et al Science. 2001 Nov 23;294(5547):1713-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATR ATR serine/threonine kinase (ATR Human)

Tags

FLAG

Expression

Mammalian

Promoter

SRa

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLX311-Cas9

Plasmid

#118018

Purpose

Constitutive expression of Cas9

Depositor

William Hahn, David Root

Publication

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Cas9

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pMRE145

Plasmid

#118497

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1316

Plasmid

#111126

Purpose

Expresses FANCA.

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA (FANCA Human)

Use

Lentiviral

Tags

HA

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Publication

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

PcUBI4-2

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-hSyn-NES-NIR-GECO1

Plasmid

#113683

Purpose

Express NIR-GECO1 preferentially in neurons

Depositor

Robert Campbell

Publication

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NES-NIR-GECO1

Use

AAV

Promoter

hSyn

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pIGAhRC

Plasmid

#112510

Purpose

human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

Depositor

Charles Miller

Publication

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

HIF1B, AhR (ARNT, AHR Human, Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Promoter

Gal1,10

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDNA2.0 6H-FLAG-TEV-KRAS G12C

Plasmid

#111848

Purpose

Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.

Depositor

Kenneth Westover

Publication

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS G12C (KRAS Human)

Tags

6xHis-tag, FLAG-tag, and TEV protease cleavage se…

Expression

Bacterial

Mutation

Changed Glycine 12 to Cysteine

Promoter

T5

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-FLAG- gate-pGK-HYG

Plasmid

#107397

Purpose

Gateway destination vector

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Tags

FLAG

Expression

Mammalian

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV.rTH.PI.Cre.SV40

Plasmid

#107788

Purpose

AAV vector expressing Cre from rTH promoter

Depositor

James M. Wilson

Has service

AAV9 and AAV Retrograde

Insert

Cre

Use

AAV

Expression

Mammalian

Promoter

rTH

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pX551-miniCMV-SpCas9

Plasmid

#107031

Purpose

AAV plasmid expressing Cas9 under control of miniCMV promoter

Depositor

Alex Hewitt

Insert

SpCas9

Use

AAV and CRISPR

Expression

Mammalian

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_LKB1

Plasmid

#108111

Purpose

Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Publication

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

LKB1 (STK11 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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