We narrowed to 24,940 results for: crispr
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Plasmid#196827PurposeExpress dpdpCas13b with AAAA mutationDepositorInsertdpspCas13b(AAAA)
Tags3XFLAGExpressionMammalianMutationK367A, D369A and K370AAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRO16Sh5
Plasmid#180076PurposeExpression of ShCAST under control of Plac with pRO1600 origin.DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k
UseSynthetic BiologyExpressionBacterialPromoterpLacAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1050R (Ubiq-dCasRx)
Plasmid#132417PurposeExpress dCasRx under Ubiquitin-63E promoterDepositorInsertdCasRx
ExpressionInsectPromoterUbiqAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OA-1050L (UASt-CasRx)
Plasmid#132418PurposeExpress CasRx under UASt promoterDepositorInsertsCasRx
UASt+hsp70
ExpressionInsectPromoterUAStAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pdCas9- AsiA_m1.1
Plasmid#158064PurposeExpressing evolved CasTA of dCas9 fused AsiA variant 1.1DepositorInsertdCas9-AsiA_m1.1
ExpressionBacterialPromoterTetOAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta 100-175
Plasmid#99667PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorPublicationInsertdCas9
Tagstruncated RTA activation domain (101-175)ExpressionMammalianMutationdead Cas9Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65(100-261)-RTA(75-190)
Plasmid#99672PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorPublicationInsertdCas9
TagsVP64-p65(101-261)-RTA(75-190)ExpressionMammalianMutationdead Cas9Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
dSp-Rta 50-175
Plasmid#99665PurposeExpresses dSp Cas9 fused to truncated RTA activation domainDepositorPublicationInsertdCas9
Tagstruncated RTA activation domain (51-175)ExpressionMammalianMutationdead Cas9Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT055f
Plasmid#96865PurposesgRNA-FANCFDepositorInsertsgRNA-FANCF
ExpressionMammalianAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWT055g
Plasmid#96864PurposesgRNA-HEK3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA-HEK3
ExpressionMammalianAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH372
Plasmid#86839Purposemamalian expression of AcrIIA1DepositorInsertacrIIA1
ExpressionMammalianPromoterCMVAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRVV651
Plasmid#87631PurposeDonor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBxb1 attP
UseCRISPRAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET_His6-SUMO-TEV-TtCsm6
Plasmid#172486PurposeBacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.DepositorInsertT. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
TagsHis-SUMO-TEVExpressionBacterialPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV/eGFP-U6-sgRNA
Plasmid#170544PurposeA lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDRDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJGL002
Plasmid#180606PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasXDepositorInsertPlmCasX
UseCRISPRTags10xHis and MBPExpressionBacterialPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a-KRAB_crRNA NC
Plasmid#176262PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleasDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsKruppel associated box domain and luciferaseMutationD917A and E1006A mutations to inactivate the endo…Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pdpy-30-miniCAFE
Plasmid#170115PurposeDpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMiniCAFE
UseCRISPRMutationD8A, HNH-truncation (Δ495–609 aa) in CjCas9PromoterDpy-30Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113702PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV, CRISPR, and Mouse TargetingTagsKASHAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-BE3-2NLS
Plasmid#119031PurposeStably express BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE3
Tags3xFlagExpressionMammalianAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113689PurposeSaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACSDepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSPromoterCytomegalo Virus(CMV)Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by