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phage-ubc-nls-ha-tdMCP-gfp

Plasmid

#40649

Depositor

Robert Singer

Article

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

tdMCP-gfp

Use

Lentiviral

Tags

EGFP, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Puro

Plasmid

#79049

Purpose

Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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S1PR5-Tango

Plasmid

#66500

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

S1PR5 (S1PR5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
DdMCU

Plasmid

#67642

Purpose

DdMCU-Flag in pACT2 vector human codon optimised

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

DdMCU

Tags

Flag tag with linker

Expression

Yeast

Promoter

ADH1

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-R1441G

Plasmid

#25082

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arginine 1441 mutated to Glycine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-UCP3_STOP

Plasmid

#161424

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

UCP3 (UCP3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLEX-MCS-ASC-GFP

Plasmid

#73957

Purpose

Express human ASC with a C-terminal EGFP tag in mammalian cells

Depositor

Christian Stehlik

Article

de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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p119 (FUGW-UbCp-rtTA3)

Plasmid

#105171

Purpose

ubiquitin C promoter drives rtTA3 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

UbCp-rtTA3

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+ Integrin beta 1b-mKate

Plasmid

#105975

Purpose

in vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Integrinbeta1b

Tags

mKate

Expression

Bacterial

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IGFBP7-COMP5AP-AviTag-9xHis

Plasmid

#157270

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IGFBP7 (IGFBP7 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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