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pRK5-FLAG-FNIP1

Plasmid

#72292

Purpose

overexpression

Depositor

David Sabatini

Publication

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP1 (FNIP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pMON-HsNMT1

Plasmid

#67475

Purpose

Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Publication

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT1 (NMT1 Human)

Expression

Bacterial

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
XW08

Plasmid

#65832

Purpose

Punc-4-QF-SL2-mcherry-3'UTR-unc-54

Depositor

Kang Shen

Publication

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

OR74A quinic acid utilization activator (qa-1F)

Tags

mCherry

Expression

Worm

Promoter

Punc-4

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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PEG-Actinin-C-sstFRET

Plasmid

#61101

Purpose

Expression of α-actinin with C-term sstFRET inserted to measure cytoskeletal stress in vivo

Depositor

Fred Sachs

Publication

Meng et al J Cell Sci. 2011 Jan 15;124(Pt 2):261-9.

Insert

α-actinin-sstFRET

Tags

sstFRET

Expression

Mammalian

Promoter

CMV

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Publication

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DDC_M_Tub_KDEL

Plasmid

#51529

Purpose

Ectopic expression of DDC with KDEL tag for CTAP

Depositor

Claus Jorgensen

Publication

Tape et al Mol Cell Proteomics. 2014 May 12.

Insert

DDC

Tags

HA and KDEL

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Lyr_M37_KDEL

Plasmid

#51530

Purpose

Ectopic expression of Lyr with KDEL tag for CTAP

Depositor

Claus Jorgensen

Publication

Tape et al Mol Cell Proteomics. 2014 May 12.

Insert

Lysine Racemase

Tags

HA and KDEL

Expression

Mammalian

Mutation

aa's 1-36 removed

Promoter

CMV

Available since

April 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBRHDAd-TKVenus

Plasmid

#26422

Depositor

Kohnosuke Mitani

Publication

Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

HSV-TK, CS2-Venus

Use

Adenoviral

Expression

Mammalian

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pEF6V5:eGFP-CAAX-2A-mCherry

Plasmid

#26901

Depositor

Steven Leach

Publication

Provost et al Genesis. 2007 Oct . 45(10):625-9.

Insert

eGFP-CAAX-PTVI-2A-mCherry

Expression

Mammalian

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCaryP

Plasmid

#18945

Depositor

Michele Calos

Publication

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 attP

Expression

Insect

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV6 Myc PAK5 WT

Plasmid

#16019

Depositor

Jonathan Chernoff

Publication

Cotteret et al Mol Cell Biol. 2003 Aug . 23(16):5526-39

Insert

PAK5 (PAK5 Human)

Tags

Myc

Expression

Mammalian

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJSX-GL3

Plasmid

#11980

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 binding site

Depositor

Ron Prywes

Publication

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEGFP-C1-wtVHL

Plasmid

#13390

Depositor

Lucy Anderson

Publication

Shiao et al Cancer Res. 2000 Jun 1. 60(11):2816-9.

Insert

von Hippel-Lindau (Vhl Rat)

Tags

GFP

Expression

Mammalian

Available since

Nov. 30, 2006

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Cebpa

Plasmid

#139834

Purpose

doxycycline-inducible expression of mouse Cebpa in mammalian cells

Depositor

Filipe Pereira

Publication

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Cebpa (Cebpa Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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SMA-Cherry

Plasmid

#110209

Purpose

Mapping of mesenchymal progenitor cells.

Depositor

David Rowe

Publication

Grcevic et al Stem Cells. 2012 Feb;30(2):187-96. doi:

Insert

mCherry

Tags

None

Expression

Mammalian

Promoter

Smooth muscle alpha-actin and first intron

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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CRY2PHR-PSD95FingR-GFP

Plasmid

#89443

Purpose

Localizes CRY2 to excitatory synapses

Depositor

Matthew Kennedy

Publication

Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

Insert

CRY2-PSD95FingR-GFP

Expression

Mammalian

Mutation

Contains CRY2 a.a. 1-498

Promoter

CAG

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g2

Plasmid

#80785

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

Depositor

Yonglun Luo

Publication

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaE (pESC124)

Plasmid

#50390

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Publication

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

DPCK

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-21a(+)/MBP-His-PINK1

Plasmid

#38005

Depositor

Michael J Fox Foundation MJFF

Insert

PINK1 (PINK1 Human)

Tags

6xHIS and MBP

Expression

Bacterial

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only
His-drebrin

Plasmid

#40362

Depositor

Phillip Gordon-Weeks

Publication

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles