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pFC34K LgBiT TK-Neo Human collagen type IV alpha 5 chain (COL4A5)

Plasmid

#229770

Purpose

Human Col4A5 tagged at C-terminal with LgBiT to be used with C-tagged SmBiT hCol4A3 and Flag-tagged hCol4A4 in Nanobit assay system

Depositor

Hirofumi Kai

Article

Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

Insert

Human collagen type IV alpha 5 chain (COL4A5) (COL4A5 Human)

Use

Luciferase

Tags

LgBiT

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

Plasmid

#229769

Purpose

Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system

Depositor

Hirofumi Kai

Article

Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64

Insert

Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)

Use

Luciferase

Tags

SmBiT

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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mRFP1-N1

Plasmid

#54635

Purpose

Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Tags

mRFP1

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Ptgs2

Plasmid

#209519

Purpose

Expresses luciferase under control of Ptgs2 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

Insert

Ptgs2 (Ptgs2 Mouse)

Use

Luciferase

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-H2B-PAmCherry

Plasmid

#133419

Purpose

Fluorescent reporter for calcium imaging and photoactivation

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pg-HIF-1alpha-EGFP

Plasmid

#87204

Purpose

C-terminally EGFP-tagged human HIF-1a

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-1a (HIF1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

L40F, M88V, Q814R

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFast-hRad1

Plasmid

#222471

Purpose

Expresses hRad1 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hRad1 (RAD1 Human)

Expression

Insect

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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CSII-prEF1a-mCherry-3xNLS

Plasmid

#125262

Purpose

Fluorescent reporter for cell size

Depositor

Jan Skotheim

Article

Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

Insert

mCherry-3xNLS

Use

Lentiviral

Tags

3xNLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAM1579

Plasmid

#40240

Depositor

Susan Golden

Article

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

Km Resistance, neutral site II integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR V7

Plasmid

#86856

Purpose

Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

Depositor

Michael Mancini, Marco Marcelli

Article

Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

Insert

Androgen receptor (AR) (AR Human)

Tags

EGFP

Expression

Mammalian

Mutation

Alternative splice variant 7 (alteration/deletion…

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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