We narrowed to 19,374 results for: INO

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pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pX330S-4

Plasmid

#58780

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGWB601NL3F10H

Plasmid

#141287

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCBL101.1

Plasmid

#199724

Purpose

A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

Multiple Cloning Site, NPTII

Expression

Plant

Mutation

"GAGACC" to "GATACC"

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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GB4068

Plasmid

#187810

Purpose

Module for the constitutive expression of Cup2:Gal4AD and copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

copper-inducible dCasEV2.1

Expression

Plant

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMB-ACTFr

Plasmid

#61192

Purpose

Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtFimbrin1-ABD

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSGKP-spe

Plasmid

#117234

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 Full Length-6MT

Plasmid

#41728

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Full Length (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

L1997V; Contains murine Notch-1 protein up to ami…

Available Since

Dec. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD_Kan-LacI

Plasmid

#79826

Purpose

Arabinose induced expression of Lac repressor

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Insert

LacI

Tags

His6

Expression

Bacterial

Promoter

araBp

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJLG029

Plasmid

#192980

Purpose

BHR plasmid (pRA301) expressing pruR-3XFLAG from T7 promoter for high yield protein purification

Depositor

Clay Fuqua

Article

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

pruR-3xFLAG

Tags

3XFLAG

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ141B2.0

Plasmid

#99897

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE15

Plasmid

#90354

Purpose

RARA - Retinoic acid receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

RARE

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Sp_PE

Plasmid

#212038

Purpose

Bacterial expression of the PE2 proteins for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

PE2

Use

CRISPR

Promoter

L-arabinose-inducible promoter PBAD

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJAT10

Plasmid

#204293

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGW-TurboID-GOI-GFP

Plasmid

#209383

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Expression

Plant

Mutation

TurboID gene sequence C249A

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA63_HelperΔgIII

Plasmid

#190433

Purpose

M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

M13 genes I-V and VII-XI

Use

Synthetic Biology

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGW-GFP-GOI-TurboID

Plasmid

#209382

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Expression

Plant

Mutation

TurboID gene sequence C249A

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-RRvT

Plasmid

#87364

Purpose

Expresses RRvT, a tandem heterodimer red fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

RRvT

Tags

His-Tag

Expression

Bacterial

Promoter

pBad (Arabinose)

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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EMTB-mCherry

Plasmid

#26742

Depositor

William Bement

Article

Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

Insert

ensconsin (MAP7 Human)

Use

Probe for microtubules

Tags

2x mCherry and Myc

Expression

Mammalian

Mutation

Microtubule binding-domain of ensconsin (amino ac…

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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