We narrowed to 23,118 results for: Sis

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pHH0103_MYO15A-2/2

Plasmid

#91291

Purpose

Protein expression and purification of human SH3 domain construct MYO15A-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO15A-2/2 (MYO15A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB525

Plasmid

#74867

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG HDAC5 S259A

Plasmid

#32214

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser259Ala

Promoter

CMV

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pEYFP-Pds1Δdestruction box(383)

Plasmid

#39848

Depositor

Jonathon Pines

Article

Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

Insert

Pds1 (PTTG1 Human)

Tags

EYFP

Expression

Mammalian

Mutation

Δdestruction box = ΔR61-N68

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (AA)

Plasmid

#24240

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AA) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Threonine 56 to Alanine changed Serine …

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pJOE5751.1

Plasmid

#135083

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-His tag-eGFP

Expression

Bacterial

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSYN-VWF-039

Plasmid

#120292

Purpose

VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein

Depositor

Robert Peters

Article

Dong et al Blood. 2019 Jan 14. pii: blood-2018-10-8

Insert

VWF D1-D2-D'-D3-Fc

Tags

Fc

Expression

Mammalian

Mutation

C1099A/C1142A

Promoter

CMV

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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FFiBlast MSCV Puro

Plasmid

#68464

Purpose

Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox and Retroviral

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB429

Plasmid

#74823

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP RanSen

Plasmid

#184705

Purpose

Lentiviral transfer plasmid for expression of GFP RanSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP RanSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_PPP1R13L-1/1

Plasmid

#91509

Purpose

Protein expression and purification of human SH3 domain construct PPP1R13L-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PPP1R13L-1/1 (PPP1R13L Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-TPI_WT-xrRNA-4H

Plasmid

#108368

Purpose

Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

Depositor

Niels Gehring

Article

Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

Insert

Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)

Plasmid

#87735

Purpose

Bacterial expression of N-terminally truncated S. cerevisiae Vps4

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-FLAG-mTUT4(D1028A)

Plasmid

#60220

Purpose

Mammalian expression of FLAG-tagged catalytic mutant of TUT4

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT4 (Tut4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

D1028A

Promoter

CMV

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pBI-CMV1-mir421-CMV2-mir1

Plasmid

#164673

Purpose

Express miR-421 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-421 (MIR421 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-MRPP1_native+

Plasmid

#67865

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form)

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP1 (TRMT10C Human)

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pJMP1102

Plasmid

#119246

Purpose

knockdown phzA1 in P. aeruginosa

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA phzA1 (P. aeruginosa)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1103

Plasmid

#119247

Purpose

knockdown phzM in P. aeruginosa

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA phzM (P. aeruginosa)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-SIK1

Plasmid

#51577

Purpose

Constitutively express nucleolus localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SIK1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-18a

Plasmid

#64228

Purpose

Expresses miR-18a in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-18a (MIR18A Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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