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HA-MBP-TEVpCS-ADGRG7 CTFdeltaTA-FLAG

Plasmid

#217738

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG7 (ADGRG7 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-421

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

Plasmid

#229828

Purpose

A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGM32DEST

Plasmid

#74747

Purpose

Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

Gateway cassette
QF-GR (NR3C1 Synthetic, Human, Neurospora crassa)

Tags

Fusion of the Neurospora crassa QF activator to t…

Expression

Worm

Promoter

No promoter

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-LBR(1-245)-GFP

Plasmid

#222425

Purpose

To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(1-245aa) (LBR Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Truncation (Includes aa 1-245)

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Fly, Human, Aequorea victoria)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pGART-2XStrep

Plasmid

#125681

Purpose

expresses human GART protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

GART (GART Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-HOXA5

Plasmid

#97040

Purpose

Expresses HOXA5 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Homeobox A5 (HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-N (TRP1)

Plasmid

#177797

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE MDER

Plasmid

#13494

Depositor

Stephen Tapscott

Article

Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

Insert

MyoD1-Estrogen Receptor Fusion Protein (Myod1 Mouse, Human)

Use

Retroviral

Expression

Mammalian

Mutation

full length MyoD was fused to aa282-595 of Esr1. …

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only

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pBZCas13b-HEPN

Plasmid

#89901

Purpose

Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.

Depositor

Feng Zhang

Article

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

Cas13b

Expression

Bacterial

Mutation

R116A/H121A/R1177A/H1182A

Promoter

Lac

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK.MD(ND2bHLH).WPRE

Plasmid

#122055

Purpose

Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain

Depositor

Stephen Tapscott

Article

Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

Insert

MD(ND2bHLH) (Myod1, Neurod2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

MyoD chimeric protein substituted with the NeuroD…

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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FKBP-beta3(702-1094)-GFP

Plasmid

#100734

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP3B1 (AP3B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

appendage and ear only

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)

Plasmid

#100742

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD8A (CD8A Human)

Tags

LOVpep and tagRFP657

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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FKBP-beta2(616-951)-mRuby2

Plasmid

#100730

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and mRuby2

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-CMV/TetO2-EYFP-GW

Plasmid

#153309

Purpose

Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Type

Empty backbone

Use

Transposon

Tags

Yellow Fluorescent Protein

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArB WT RNAiR

Plasmid

#59806

Purpose

Allows the integration of Myc ArB in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora B (AURKB Human)

Tags

Myc

Expression

Mammalian

Mutation

RNAi resistant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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SBC009876

Plasmid

#226283

Purpose

Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

Depositor

Nigel Scrutton

Article

Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1

Expression

Bacterial

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG

Plasmid

#163719

Purpose

Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG

Expression

Bacterial

Mutation

dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBullet-tgn-c

Plasmid

#53072

Purpose

destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRA3 CTF-FLAG

Plasmid

#217680

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRA3 (ADGRA3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-737

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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