We narrowed to 22,456 results for: his
-
Plasmid#98229PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJAK3A (JAK3 Human)
UseBaculovirusTagsHis-6-TEVMutationpfam00069:Pkinase 822-1091PromoterpolyhedrinAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
Tags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
Tags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT16
Plasmid#89659Purposeexpression of doubleTAL staple protein dT16 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT16
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT17
Plasmid#89660Purposeexpression of doubleTAL staple protein dT17 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT17
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT21
Plasmid#89662Purposeexpression of doubleTAL staple protein dT21 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT21
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT32
Plasmid#89667Purposeexpression of doubleTAL staple protein dT32 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT32
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT-HA-STX1
Plasmid#89310PurposeExpresses HA tagged syntaxin 1 in Drosophila cellsDepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP4.3.2
Plasmid#98270PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.2.2
Plasmid#97357PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET MBP tdTomato LIC cloning vector (MBP-tdTomato)
Plasmid#86927PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-tdTomatoDepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialPromoterT7Available SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
RGD-sfGFP-I27-CC
Plasmid#85392PurposeMTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labelingDepositorInsertRGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
Tags6x HisExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTlpA-Wasabi-NF
Plasmid#86100PurposeNonfluorescent variant of mWasabi reporter gene gated by wild type TlpADepositorInsertsTlpA
mWasabi
Tags6xHisExpressionBacterialMutationD341A and S71T, G73APromoterTlpA and TlpA, LacIAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE30-rSyntenin1
Plasmid#89428Purposebacterial expression of rat Syntenin1DepositorInsertSDCBP
TagsHisExpressionBacterialPromoterT5Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS248
Plasmid#87354PurposeS867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/C574S/S867C/N1054CPromoterT7Available SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM34
Plasmid#89156PurposeRecombinant expression of PTBP1-RRM34 in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
Tagshexa HisExpressionBacterialMutationcontains RNA Recognition Motif 3+4PromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1CCtoSS
Plasmid#89157PurposeRecombinant expression of PTBP1 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
Tagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Akt3 WT-2X Flag
Plasmid#87607Purposeexpresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 proteinDepositorInsertAkt3 isoform 2 (AKT3 Human)
UseZebrafish (danio rerio) expressionTags2 tandem flag tags and His-Halo-TevExpressionMammalianMutationAkt3 isoform 2PromoterSP6Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)/FbGH30
Plasmid#86463PurposeExpression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coliDepositorInsertFbGH30
TagsHis-TagExpressionBacterialPromoterT7Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDnaK-Wasabi
Plasmid#86110PurposemWasabi reporter gene driven by DnaK promoterDepositorInsertmWasabi
Tags6xHisExpressionBacterialPromoterpDnaKAvailable SinceFeb. 25, 2017AvailabilityAcademic Institutions and Nonprofits only