We narrowed to 31,132 results for: PLE
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Plasmid#203226PurposeFor protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
TagsN-terminal TEV cleavable 6xHis tagExpressionBacterialAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Skp2
Plasmid#19946DepositorAvailable SinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
PM-ABKAR
Plasmid#61506PurposeExpression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequenceDepositorInsertAMPK Biosensor
TagsLyn kinase sequence (see comments)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10333
Plasmid#183955PurposeCAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertNuclease-dead Lb Cas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832APromoterCAGAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (T1462A)
Plasmid#14130DepositorAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
NET33 pEGFP-N2 (597)
Plasmid#61989Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG-FFLuc-3XFLAG
Plasmid#127333PurposeExpress 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codonDepositorInsertFirefly luciferase
Tags3xFLAGExpressionMammalianPromoterCV40Available SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
-2.5Kb MMP10
Plasmid#53973PurposeLuciferase reporter for mouse MMP10 2.5kb promoterDepositorInsertmouse MMP10 2.5Kb promoter (Mmp10 Mouse)
UseLuciferaseExpressionMammalianPromoterPCR from C57BL6/JAvailable SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDGE496
Plasmid#153248PurposeM6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE495
Plasmid#153247PurposeM5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 hBRM
Plasmid#1956DepositorAvailable SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG RUNX3 (R122C)
Plasmid#203438PurposeMammalian expression of RUNX3DepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW149
Plasmid#122248PurposepTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.DepositorInsertTIF4631 (TIF4631 Budding Yeast)
TagsIntein-Chitin-binding domainExpressionBacterialPromoterT7Available SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-GOLGA4-GRIP
Plasmid#220068Purposeoverexpreeses the TGN protein GRIP with a mCherry fluorophoreDepositorInsertGOLGA4-GRIP (GOLGA4 Human)
ExpressionMammalianAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-HIV-1 Group P-vpr
Plasmid#115837PurposeEncodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance proteinDepositorInsertHIV1 Group P Vpr (HQ179987)
UseLentiviralTags3x-FLAGPromoterSFFVAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR(T/A)
Plasmid#118469PurposeNegative-control mutant for sapphireCKAR biosensor.DepositorInsertsapphireCKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-POC5
Plasmid#136786PurposeMammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tagDepositorInsertSNAP-POC5 (POC5 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
(SIM)5
Plasmid#126947PurposeBacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
ExpressionBacterialPromoterunknownAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-Sec24D
Plasmid#66614PurposeYFP fusion of Sec24DDepositorAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE341
Plasmid#153250PurposeM7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only