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pAdEasy-EGFP-CLASP2 340-1084

Plasmid

#24524

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1084) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pISH-Gnas

Plasmid

#105483

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Gnas (Gnas Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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RBMS3_2xRRM_pGEX

Plasmid

#135142

Purpose

Recombinant expression of RBMS3 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBMS3 (RBMS3 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

July 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (E542K)

Plasmid

#29530

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed glutamic acid 542 to lysine

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pFUGW-GFP-2-cut reporter

Plasmid

#131669

Purpose

Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

GFP-2-cut reporter

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Promoter

EFS-NS

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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MTK8a_005

Plasmid

#123889

Purpose

Encodes the F1 Kanamycin resistance cassette as a type 8a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-KanR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK7_012

Plasmid

#123879

Purpose

Encodes the 5' 500 bp hCYBYL locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hCLYBL

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8b_002

Plasmid

#123891

Purpose

Encodes the 3' 500 bp hRosa26 locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_hROSA26

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK8b_010

Plasmid

#123897

Purpose

Encodes the 3' 500 bp hCLYBL locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_hCLYBL

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_030

Plasmid

#123910

Purpose

Encodes the spCas9 sgRNA1 hCLYBL (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 -hCLYBL

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK7_002

Plasmid

#123871

Purpose

Encodes the 5' 500 bp hRosa26 locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hROSA26

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK7_011

Plasmid

#123878

Purpose

Encodes the 5' 500 bp hThal4.10-2-gh18 locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hTha5.10-2-hg18

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK6_003

Plasmid

#123857

Purpose

Encodes pCMV-Blasticidin resistance cassette as a type 6 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

CMV-Blast-bgPA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_014

Plasmid

#123677

Purpose

Encodes ConLS' and short flippase recognition target (FRT) site as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS'-FRT

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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RBM24_RRM_pGEX

Plasmid

#135148

Purpose

Recombinant expression of RBM24 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM24 (RBM24 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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(PM-S608E)FLAG-Cep215-FL

Plasmid

#106906

Purpose

Expresses murine phospho-mimetic CEP215 at S608

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

CEP215 (Cdk5rap2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine-608 to Glutamic Acid

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847

Plasmid

#91914

Purpose

Expresses CgCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

CgCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD2_3

Plasmid

#36370

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD2 (MBD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pGEM mGata3

Plasmid

#25796

Depositor

Douglas Engel

Article

Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

Insert

Gata3 (Gata3 Mouse)

Expression

Bacterial

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (E317K)

Plasmid

#29537

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only