We narrowed to 617 results for: spen

1. pGL3-noSV40-humanEC1.45_p300peak1-2_del8

   Plasmid

   #173980

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #8
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Tiled deletion #8

   Promoter

   SV40 promoter

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGG442

   Plasmid

   #165616

   Purpose

   Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
   

   Use

   CRISPR

   Tags

   3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

   Expression

   Yeast

   Mutation

   Corrected homology relative to wild type SpCas9 (…

   Promoter

   TEF1

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458-sgEIF4EBP1

   Plasmid

   #128097

   Purpose

   CRISPR gRNA against human EIF4EBP1 (4EBP1) with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   CRISPR sgRNA against human 4EBP1 (EIF4EBP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.22-PGK1-HRE::dLUC

   Plasmid

   #128095

   Purpose

   Hypoxia response element real-time luciferase reporter

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
   

   Use

   Luciferase

   Tags

   drives expression of destabilized luciferase (2CP…

   Expression

   Mammalian

   Mutation

   Promoter

   inserted PGK1 hypoxia response elements (HRE) (x3)

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC763 - pX458 with rat Rosa26 gRNA A

   Plasmid

   #113161

   Purpose

   A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat Rosa26
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

   Plasmid

   #113163

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS/XBB.1.16-RBD

   Plasmid

   #213070

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/XBB.1.16-RBD (S )
   

   Use

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pαH-S-GSAS-2P-Omicron-BA.5

   Plasmid

   #213828

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pαH-S-GSAS/Omicron-2P-BA5 (S )
   

   Use

   Tags

   HRV 3C cleavage site (before tags) 8X His tag 2X…

   Expression

   Mammalian

   Mutation

   G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRM14

   Plasmid

   #163693

   Purpose

   hsp70 promoter inducible expression of DHB:mNeonGreen-P2A-H2B:mScarlet

   Depositor

   David Matus

   Article

   Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

   Insert

   DHB:mNeonGreen-P2A-H2B:mScarlet (HELB, H2BC11 Human)
   

   Use

   Unspecified; Zebrafish expression

   Tags

   mNeonGreen and mScarlet

   Expression

   Mutation

   Promoter

   HSP70

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

    Plasmid

    #112677

    Purpose

    An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Nuclear-localized floxed-mCherry EGFP
    

    Use

    AAV

    Tags

    Nuclear localization signal

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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