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pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

Plasmid

#184740

Purpose

encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109

Depositor

Randall Platt

Article

Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

Insert

FsRT-Cas1(opt)-Cas2(opt)

Use

Tags

Expression

Bacterial

Mutation

sequence codon optimized for expression in E. coli

Promoter

pTetA

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pKSS

Plasmid

#39272

Depositor

Peter Kast

Article

Kast et al Gene. 1994 Jan 28;138(1-2):109-14.

Type

Empty backbone

Use

Positive-selection cloning vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only

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Recall-miniCMV-sfGFP

Plasmid

#137995

Purpose

Backbone for generation of ClonMapper Recall Plasmid

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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5XDRE-luciferase

Plasmid

#85623

Purpose

Luciferase reporter containing five tandem copies of the core Dpp response element

Depositor

Kenneth Irvine

Article

Oh et al Dev Cell. 2011 Jan 18;20(1):109-22. doi:

Insert

5x DRE

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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HA.PKD

Plasmid

#10808

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

L520P

Promoter

CMV

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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HA.PKD.Y463F

Plasmid

#10813

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Y463F, inactive

Promoter

CMV

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pLVPT-rtTR-KRAB

Plasmid

#11777

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB, Tet-off

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKD1.RNAi

Plasmid

#10815

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD1 RNAi (PRKD1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pLVU-tTR-KRAB

Plasmid

#11645

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, no tetO

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
ExRai-PKNAR

Plasmid

#226631

Purpose

Excitation-ratiometric biosensor for monitoring Protein Kinase Novel activity in living cells

Depositor

Ju Chen

Article

Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

Insert

ExRai-PKNAR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Crop-Seq-BFP-WPRE-TS-hU6-BsmbI

Plasmid

#137993

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in droplet-based sequencing

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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PM-ExRai-PKNAR

Plasmid

#226633

Purpose

Plasma membrane-targeted ExRai-PKNAR

Depositor

Ju Chen

Article

Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

Insert

PM-ExRai-PKNAR

Use

Tags

Expression

Mammalian

Mutation

Plasma membrane targeting N-terminal 11 amino aci…

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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PM-ExRai-PKNAR(T/A)

Plasmid

#226634

Purpose

Negative-control mutant for PM-ExRai-PKNAR biosensor

Depositor

Ju Chen

Article

Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

Insert

PM-ExRai-PKNAR(T/A)

Use

Tags

Expression

Mammalian

Mutation

Phosphorylatable Thr of PM-ExRai-PKNAR mutated to…

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Use

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

Plasmid

#137994

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM60-Tia1

Plasmid

#38243

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Tia1 (Tia1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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HA.PKD.Y463E

Plasmid

#10812

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Y463E--active; P467L and L520P

Promoter

CMV

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pLJM60-Tiar

Plasmid

#38244

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Tiar (Tial1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
ExRai-PKNAR(T/A)

Plasmid

#226632

Purpose

Negative-control mutant for ExRai-PKNAR biosensor

Depositor

Ju Chen

Article

Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

Insert

ExRai-PKNAR (T/A)

Use

Tags

Expression

Mammalian

Mutation

Phosphorylatable Thr mutated to Ala

Promoter

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLVCT-tTR-KRAB

Plasmid

#11643

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

CAG promoters, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge