We narrowed to 9,223 results for: Coli

1. pHL 802

   Plasmid

   #52991

   Purpose

   pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   OxyS sRNA
   ompC
   

   Use

   Synthetic Biology

   Tags

   GFP and RBS-mCherry

   Expression

   Bacterial

   Mutation

   contains −81 to +36 bp relative to start codon

   Promoter

   pLlacO-1 and pLtetO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pHL 1020

   Plasmid

   #52995

   Purpose

   pLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   DsrA sRNA (competitor)
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp

   Expression

   Bacterial

   Mutation

   contains −56 to +141 bp relative to start codon

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pHL 1019

   Plasmid

   #52994

   Purpose

   pLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   MicC sRNA (Competitor)
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp

   Expression

   Bacterial

   Mutation

   contains −56 to +141 bp relative to start codon

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pHL 1039

   Plasmid

   #53000

   Purpose

   pLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   DsrA sRNA
   OxyS sRNA (competitor)
   rpoS
   

   Use

   Synthetic Biology

   Tags

   gfp

   Expression

   Bacterial

   Mutation

   contains −150 to +30 bp relative to start codon

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pHL 1073

   Plasmid

   #53003

   Purpose

   pCon:ompC::mCherry, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   mCherry
   GFP
   

   Use

   Synthetic Biology

   Tags

   ompC (target mRNA) and sodB (target mRNA)

   Expression

   Bacterial

   Mutation

   contains −56 to +141 bp relative to start codon a…

   Promoter

   pCon and pLlacO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pHL 1303

   Plasmid

   #53008

   Purpose

   pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   sodB
   ompC
   

   Use

   Synthetic Biology

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Mutation

   contains -56 to +141 relative to start codon and …

   Promoter

   pLlacO-1 and pLtetO-1

   Available since

   May 14, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pExp-Trx

   Plasmid

   #112577

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-TrxA and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pExp-MsyB

   Plasmid

   #112570

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8His and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

   Plasmid

   #55209

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   10xAsn, 10xHis, MPB, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 MBP TEV LIC cloning vector (2M-T)

    Plasmid

    #29708

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

    Plasmid

    #29722

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pExp-TF

    Plasmid

    #112575

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-TriggerFactor and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pExp-DsbC

    Plasmid

    #112564

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-DsbC and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. INT_Cargo_PgyrA_gfp

    Plasmid

    #212138

    Purpose

    Cargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.

    Depositor

    Kang Zhou

    Insert

    green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2

    Plasmid

    #177231

    Purpose

    Expresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinase

    Depositor

    Monte Winslow

    Insert

    sgNeo1/sgNT1/sgNeo2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    bU6/mU6/hU6

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid

    #86928

    Purpose

    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and td…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pxCas9CR4

    Plasmid

    #111656

    Purpose

    PCas9CR4 with the xCas9 mutations allowing NG PAM sites

    Depositor

    Christopher Reisch

    Insert

    TetR and xCas9
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    xCas9 mutations

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOliSpec5'

    Plasmid

    #228551

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 5' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOliSpec3'

    Plasmid

    #228550

    Purpose

    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSpec5'

    Plasmid

    #228549

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 5' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSpec3'

    Plasmid

    #228548

    Purpose

    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

    Depositor

    Jeferson Gross

    Insert

    Truncated 3' region of aadA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. INT_Cargo_T7_gutB

    Plasmid

    #212139

    Purpose

    Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.

    Depositor

    Kang Zhou

    Insert

    xylitol dehydrogenase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCT03

    Plasmid

    #212150

    Purpose

    Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.

    Depositor

    Kang Zhou

    Insert

    alanine dehydrogenase
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pExp-TFSumo

    Plasmid

    #112576

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-TFSumo and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pExp-Tsf

    Plasmid

    #112578

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-Tsf and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHiKdtA

    Plasmid

    #36269

    Depositor

    Christian Raetz

    Article

    Chung et al Biochemistry. 2010 May 18;49(19):4126-37

    Insert

    H. influenzae KdtA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araC

    Available since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pBAD-WzxE-TEV-His10

    Plasmid

    #226781

    Purpose

    Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

    Depositor

    James Naismith

    Article

    Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

    Insert

    wzxE
    

    Use

    Tags

    TEV cleavable 10xHistidine tag

    Expression

    Bacterial

    Mutation

    valine insertion in position 2 due to the cloning…

    Promoter

    araBAD

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTW5126

    Plasmid

    #129732

    Purpose

    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

    Insert

    eatA-DUF::His8
    

    Use

    Tags

    9His tag

    Expression

    Bacterial

    Mutation

    replaces the domain of unknown function correspon…

    Promoter

    araBAD

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge