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pEGFP-N1-1x

Plasmid

#172281

Purpose

Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-1x

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-PpAPOBEC1 R33A

Plasmid

#138345

Purpose

Mammalian expression plasmid for BE4-PpABOBEC1 R33A

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-PpAPOBEC1 R33A

Use

Synthetic Biology

Tags

BP-NLS

Expression

Mutation

Change of Arg 33 to Ala.

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus

Plasmid

#25734

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-C–dSpCas9

Plasmid

#157834

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK-Zeo

Plasmid

#25736

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEJS2423_Nme2.Smu-ABE8e-i7

Plasmid

#201521

Purpose

Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i7

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2.Smu-ABE8e-i7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2164_Nme2-ABE8e-i7

Plasmid

#201517

Purpose

Mammalian expression of domain inlaid Nme2-ABE8e-i7

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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miniABEMax V82G wt

Plasmid

#135362

Purpose

Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

miniABEMax nSpyCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS2422_Nme2.Smu-ABE8e-i1

Plasmid

#201520

Purpose

Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i1

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2.Smu-ABE8e-i1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2424_Nme2.Smu-ABE8e-i8

Plasmid

#201522

Purpose

Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i8

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2.Smu-ABE8e-i8

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2156_Nme2-ABE8e-nt

Plasmid

#201503

Purpose

Mammalian expression of N-terminally fused Nme2-ABE8e-nt

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-nt

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2158_Nme2-ABE8e-i1

Plasmid

#201510

Purpose

Mammalian expression of domain inlaid Nme2-ABE8e-i1

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2437_Nme2.Smu-ABE8e-nt

Plasmid

#201519

Purpose

Mammalian expression of N-terminally fused Nme2.Smu-ABE8e-nt

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2.Smu-ABE8e-nt

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2165_Nme2-ABE8e-i8

Plasmid

#201518

Purpose

Mammalian expression of domain inlaid Nme2-ABE8e-i8

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i8

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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epi-BE4max-NG

Plasmid

#135977

Purpose

Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only
pJY-RpABE-PDS3_IMS

Plasmid

#112875

Purpose

binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

Depositor

Jae-Young Yun, Jin-Soo Kim

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA targeting PDS3

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

Plasmid

#200401

Purpose

Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

Open-loop DART VADAR sensor entry cassette

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-N–dSpCas9

Plasmid

#157835

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFBP-2_6.sensor

Plasmid

#162800

Purpose

Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

2_6_RNA-device

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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