We narrowed to 24,593 results for: Nes

1. UbC-Halo-SFPQY527A

   Plasmid

   #166947

   Purpose

   Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ-Y527A (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   Halo

   Expression

   Mutation

   SFPQ-Y527A

   Promoter

   UbC

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. KLC1N343S-Myc

   Plasmid

   #166963

   Purpose

   Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KLC1-N343S (Klc1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   KLC1-N343S

   Promoter

   CMV

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM33_Nsp3c_SUD-C

   Plasmid

   #156471

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3c_SUD-C (ORF1ab Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM33_Nsp3e_NAB

   Plasmid

   #156473

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3e_NAB (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

   Plasmid

   #139096

   Purpose

   Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dxCas9 3.7
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. DLG3_PDZ_2

   Plasmid

   #103947

   Purpose

   Protein expression and purification of NE-DLG PDZ2 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   DLG3 (DLG3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-GRAB_NE1m

   Plasmid

   #123308

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based NE sensor GRAB_NE1m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   contains a glycine-to-threonine mutation at posit…

   Promoter

   hSyn

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti PGK mKate2-OGT K842M (Puro)

   Plasmid

   #154291

   Purpose

   Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x FLAG and mKate2

   Expression

   Mammalian

   Mutation

   K842M mutation (Catalytic Inactive)

   Promoter

   PGK

   Available Since

   Dec. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-hACE2-hygro

   Plasmid

   #161758

   Purpose

   Expresses human ACE2 in mammalian cells

   Depositor

   Neville Sanjana

   Article

   Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Nov. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pHR_PGK-hACE2

    Plasmid

    #161612

    Purpose

    Expression of human ACE2 in mammalian cells

    Depositor

    Brad Rosenberg

    Article

    Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

    Plasmid

    #181973

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR

    Plasmid

    #181972

    Purpose

    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti PGK mKate2-OGT D554N (Puro)

    Plasmid

    #154292

    Purpose

    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x FLAG and mKate2

    Expression

    Mammalian

    Mutation

    D554N mutation (Ser/Thr glycosylation-incompetent)

    Promoter

    PGK

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SBC009876

    Plasmid

    #226283

    Purpose

    Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

    Depositor

    Nigel Scrutton

    Article

    Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

    Inserts

    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti PGK mKate2-OGT 5N5A (Puro)

    Plasmid

    #154293

    Purpose

    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x FLAG and mKate2

    Expression

    Mammalian

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations; HCF-…

    Promoter

    PGK

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-KIF5A tail only

    Plasmid

    #166956

    Purpose

    Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    KIF5A (820-1027aa)

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX307_PDHA2S293A

    Plasmid

    #115197

    Purpose

    Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PDHA2S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    p.S293A

    Promoter

    EF1alpha

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-KIF5A delta Tail

    Plasmid

    #166955

    Purpose

    Expresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    KIF5A (1-820aa)

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-crRNA(TARDBP)

    Plasmid

    #109323

    Purpose

    AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TARDBP

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH (TARDBP Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn1

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX307_PDHA2

    Plasmid

    #115192

    Purpose

    Lentiviral transduction and expression of PDHA2 into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PDHA2WT (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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