Addgene

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plentiCRISPR-v2 Puro SPNS1_sg1

Plasmid

#218530

Purpose

sgRNA targeting human SPNS1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

SPNS1 (SPNS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro CHKA_sg2

Plasmid

#218523

Purpose

sgRNA targeting human CHKA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CHKA (CHKA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v1 GFP FLVCR1_sg5

Plasmid

#218521

Purpose

sgRNA targeting human FLVCR1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-HA (in pLEX_307)

Plasmid

#214918

Purpose

constitutive expression of SRRD tagged with HA

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCriprV2-sgRNA-CRY2-#1

Plasmid

#189989

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hCRY2, works with Addgene 189983-189986

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Cryptochrome-2 (CRY2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCriprV2-sgRNA-PER1-#2

Plasmid

#189988

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Period1 (PER1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 hygro sgUCK1

Plasmid

#211524

Purpose

Deletes UCK1

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgUCK1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-ZNF746

Plasmid

#207822

Purpose

Dual expression of Cas9 and sgRNA targeting ZNF746

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746 sgRNA (ZNF746 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

EF-1a; U6

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-A-a2-GEMS(PLCG)

Plasmid

#209114

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (PLCG)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a2-GEMS(PLCG)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP10B_G671R/N865K

Plasmid

#204478

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B G671R/N865K mutants

Depositor

Veerle Baekelandt

Article

Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

Insert

ATP10B (ATP10B Human)

Use

Lentiviral

Tags

Expression

Mutation

G671R / N865K

Promoter

CMV

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-DCK-Hygro

Plasmid

#202409

Purpose

gRNA expression vector for DCK

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

guide RNA (DCK Human)

Use

CRISPR and Lentiviral

Tags

Dck

Expression

Mutation

Promoter

U6

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-MTS-jAspSnFR3

Plasmid

#203487

Purpose

Mitochondrial directed jAspSnFR3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Lentiviral

Tags

Mitochondrial localization signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti4/V5-DEST-AERRIE

Plasmid

#204023

Purpose

Lentiviral vector expressing human long non-coding RNA AERRIE (linc01013)

Depositor

Reinier Boon

Article

Pham et al Front Cell Dev Biol. 2021 Jan 11;8:61907

Insert

long non-coding RNA AERRIE (LINC01013 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-bleo-ErbB4

Plasmid

#197353

Purpose

A knock-out vector for dog ErbB4.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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mApple-TalinN3-FRB(wt)

Plasmid

#202384

Purpose

mApple-Talin(1-911)-FRB

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

mApple-Talin(1-911)-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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mApple-TalinN10-FRB

Plasmid

#202380

Purpose

mApple-Talin(1-1973)-FRB

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

mApple-Talin(1-1973)-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-mTurquoise2

Plasmid

#191010

Purpose

Lentiviral expression vector for mTurquoise2

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

mTurquoise2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1N-mApple-LZ-LOV2

Plasmid

#197418

Purpose

Mammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2022 Mar 14;32(5):1197-1205.e

Insert

MAPRE1 (MAPRE1 Human)

Use

Tags

A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

Expression

Mammalian

Mutation

EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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plex_306-ccdB-iRFP713

Plasmid

#201132

Purpose

pLEX_306 plasmid with iRFP713 marker

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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plex_307-ccdB-EYFP

Plasmid

#201146

Purpose

pLEX_307 plasmid with EYFP marker

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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