We narrowed to 4,416 results for: Pet2

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pET28a-TEV-U1AF37MF77M

Plasmid

#168267

Purpose

Expresses human dmU1A with the F37M/F77M double mutant

Depositor

Joseph Wedekind

Article

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

U1A RRM1 crystallization module (SNRPA Human)

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F37M and F77M

Promoter

T7 promotor

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET23-CBD-(-PP)-EGFP

Plasmid

#12597

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

Cdc42 Biosensor (WAS Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

F271C

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pET26-Ufd1-His

Plasmid

#21266

Depositor

Hemmo Meyer

Article

Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

Insert

Ufd1 (Ufd1l Mouse)

Tags

His

Expression

Bacterial

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_DeltaN24

Plasmid

#214327

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

Deletion of first 24 amino acids

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-5-enzymatic-domain

Plasmid

#214306

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP5 (CASP5 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

aa133-434

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-41

Plasmid

#172757

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-41

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-pro-sumo2

Plasmid

#25102

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO2 (SUMO2 Human)

Tags

His

Expression

Bacterial

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pET29b-Ava3858-3856

Plasmid

#45745

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3858-3856

Expression

Bacterial

Promoter

T7

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pET28-iLOV-Rsn2

Plasmid

#61687

Purpose

bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV

Depositor

Brian Smith

Insert

Ranaspumin-2

Tags

His and iLOV

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-GFP-CPDSalI

Plasmid

#38257

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Insert

eGFP

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

cloned from pEGFPN3 (Clonetech)

Promoter

T7

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
RanBP2-RB3-4-pET23a

Plasmid

#85769

Purpose

bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

Depositor

Frauke Melchior

Article

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Tags

His

Expression

Bacterial

Mutation

fragment (amino acids 2304-3062)

Promoter

T7

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-LSSmCherry1(-13)

Plasmid

#219415

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

LSSmCherry1(-13)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

K137E, K199E

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-miniR-DBCO

Plasmid

#181984

Purpose

Used to label proteins with the MiniE genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

MiniR-DBCO

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET21b-LaG9(S151C)

Plasmid

#172490

Purpose

Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

Depositor

Siegfried Musser

Article

Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

Insert

LaG9(S151C)

Tags

6xHis, PPX cleavage site, and pelB leader sequenc…

Expression

Bacterial

Mutation

S151C

Promoter

T7

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-26

Plasmid

#172751

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-26

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-11

Plasmid

#172762

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-11

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-Ava3858-3855

Plasmid

#45746

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3858-3855

Expression

Bacterial

Promoter

T7

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pET28b-ETV6-ETS

Plasmid

#111180

Purpose

Minimal ETS domain of human ETV6 (TEL)

Depositor

Gregory Poon

Article

Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

Insert

ETV6 ETS domain (ETV6 Mouse, Bovine, Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-L713F

Plasmid

#174794

Purpose

Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-C125G

Plasmid

#174792

Purpose

Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-C125G (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C125G, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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