We narrowed to 9,675 results for: SUB;

1. pECE-M2-AMPKalpha2 wt

   Plasmid

   #31652

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)
   

   Tags

   M2

   Expression

   Mammalian

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

2. MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG

   Plasmid

   #81022

   Purpose

   Cancer-associated ASXL1 truncation mutant in retroviral vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   p.Y591X truncation

   Promoter

   5’LTR

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCold I-Hero11

   Plasmid

   #187928

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero11

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero11 (C19orf53 Human)
   

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

   Plasmid

   #74261

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   p.Y591X truncation

   Promoter

   CMV

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

   Plasmid

   #113953

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, I132S, S212N, and …

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW TetO HA-AMPKa1 AS

   Plasmid

   #69825

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   minimal CMV promoter with tetracycline operator

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-CsChR-GFP

   Plasmid

   #58841

   Purpose

   AAV expression of CsChR-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CsChR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Syn

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCold I-Hero13

   Plasmid

   #187929

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero13

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero13 (BEX3 Human)
   

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG

   Plasmid

   #74263

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   p.R404X truncation

   Promoter

   CMV

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. GST-SF3B2 wild type aa 401-550 fragment

    Plasmid

    #67615

    Purpose

    bacterial expression of wild type GST-SF3B2 fragment (401-550)

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    splicing factor 3b subunit 2 (SF3B2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment of amino acids 401-550

    Promoter

    tac

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-SF3B2 R508K aa 401-550 fragment

    Plasmid

    #79674

    Purpose

    bacterial expression of GST-SF3B2 fragment (401-550) with R508L methylation site mutation

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    splicing factor 3b subunit 2 (SF3B2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 451-550 with R508K

    Promoter

    tac

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

12. FUW-M2-PPP1R12C S452A

    Plasmid

    #31679

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    Protein Phosphatase 1 Regulatory Subunit 12C S452A (PPP1R12C Human)
    

    Use

    Lentiviral

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 452 to Alanine

    Available Since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pBX_RPS8_smRuby2_HA

    Plasmid

    #236089

    Purpose

    Constitutively encodes RPS8-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting RPS8 in the 40S subunit.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    RPS8-smRuby2-HA (RPS8 Human, Synthetic)
    

    Expression

    Mammalian

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

    Plasmid

    #218461

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )
    

    Expression

    Mammalian

    Mutation

    Thr1273AGlu substitution

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

    Plasmid

    #218462

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )
    

    Expression

    Mammalian

    Mutation

    Thr1273Asp substitution

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. cacna2d1 R241A pMT2

    Plasmid

    #206110

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding

    Depositor

    Annette Dolphin

    Article

    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Expression

    Mammalian

    Mutation

    R241A

    Promoter

    Ad MLP/TPL/SV40

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-INTS3-C

    Plasmid

    #128642

    Purpose

    Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 C-termini (INTS3 Human)
    

    Tags

    His tag on N and C-terminal

    Expression

    Bacterial

    Mutation

    INTS3 C-termini (514–1042 aa)

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCH1/RAD51o

    Plasmid

    #102562

    Purpose

    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

    Depositor

    Maria Spies

    Article

    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

    Inserts

    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
    E. coli groE operon
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli Expression

    Promoter

    T7

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBridge-tyrosinase.σ2

    Plasmid

    #197415

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-2 σ2
    

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    encodes R42G substitution, contains silent substi…

    Promoter

    ADH1 and MET25

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6-His-MBP-TEV-FnCpf1

    Plasmid

    #90094

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 (humanized)
    

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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