We narrowed to 10,457 results for: T7

1. pBP-T7_RBS

   Plasmid

   #72978

   Purpose

   T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   T7+RBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET23a-MmUBB(SnaBI)

   Plasmid

   #69558

   Purpose

   In vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (Ubb Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The two last codons (TATTAA; encoding the last ty…

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pET23a-MmUBB(Y305P)

   Plasmid

   #69557

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (Ubb Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon TAT encoding tyrosine 305 was changed to CC…

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLEW13

   Plasmid

   #24007

   Depositor

   George Cross

   Insert

   T7 RNA Polymerase
   

   Use

   Trypanosome expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b(+)-HP0377

   Plasmid

   #39334

   Depositor

   SeWon Suh

   Insert

   oxidoreductase
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCIOX

   Plasmid

   #51300

   Purpose

   E.coli expression vector for His8-SUMO tagged recombinant protein

   Depositor

   Andrea Mattevi

   Type

   Empty backbone

   Use

   Tags

   His8 + SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVTR(T7A)-E3

   Plasmid

   #172305

   Purpose

   In vitro transcription of E3

   Depositor

   Patrik Verstreken

   Insert

   E3
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter A-initiating

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIVTR(T7A)-K3

   Plasmid

   #172306

   Purpose

   In vitro transcription of K3

   Depositor

   Patrik Verstreken

   Insert

   K3
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter A-initiating

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIVTR(T7A)-B18R

   Plasmid

   #172304

   Purpose

   In vitro transcription of B18R

   Depositor

   Patrik Verstreken

   Insert

   B18R
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter A-initiating

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIVTR(AG)-TDsmURFP

    Plasmid

    #172307

    Purpose

    In vitro transcription of TDsmURFP

    Depositor

    Patrik Verstreken

    Insert

    TDsmURFP
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter A-initiating

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAcGFP-IVT

    Plasmid

    #205145

    Purpose

    IVT template of AcGFP with MCS in frame and polyA

    Depositor

    Igor Ulitsky

    Insert

    AcGFP1
    

    Use

    In-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLNT-SFFV-mRuby2

    Plasmid

    #87217

    Purpose

    lentiviral expression of mRuby2

    Depositor

    Violaine Sée

    Insert

    mRuby2
    

    Use

    Lentiviral

    Tags

    6xHis-T7

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBP-T7_RBS-His6-Thrombin

    Plasmid

    #72979

    Purpose

    T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    T7+RBS+His6+thrombin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pJAB988

    Plasmid

    #133920

    Purpose

    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

    Depositor

    Christopher Voigt

    Article

    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.

    Insert

    miniICE-tet(M)cat(PC194)_PT7-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Worm

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2,0_3M5M_eGFP

    Plasmid

    #135474

    Purpose

    Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    Marburg virus minigenome, eGFP reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    T7

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEMHE-mCherry-mTrim21

    Plasmid

    #105522

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MC177VSG221RNAi

    Plasmid

    #59730

    Purpose

    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

    Depositor

    Gloria Rudenko

    Article

    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

    Insert

    VSG221 RNAi fragment
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    opposing T7 promoters

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Flag-H3.3-polyA

    Plasmid

    #59780

    Purpose

    Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    H3F3B (H3-3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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