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M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Promoter

hU

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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hTRAF2pLPCX-HA-Flag/P874

Plasmid

#20229

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

unmutated NM_021138.3. L replaces M at beginning…

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pFPL-Ch

Plasmid

#61647

Purpose

Gateway vector for generating cyan fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced cyan fluorescent protein

Expression

Plant

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pME-Gal4

Plasmid

#53366

Purpose

full-length native Gal4 with stop in Gateway middle entry clone

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

native (full-length) Gal4

Promoter

no promoter

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pFUGW NLS-Cre

Plasmid

#183425

Purpose

Lentiviral Cre vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

NLS-Cre

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKAR4α

Plasmid

#119919

Purpose

Mammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imaging

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKAR4α

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1E(sfGFP)

Plasmid

#126982

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_TFAP2a_enh_249_Cerulean

Plasmid

#130615

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

TFAP2a_enh_249

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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[pSK600] pRK5 HA-Nprl2(R78A)

Plasmid

#136146

Purpose

mammalian expression of Nprl2

Depositor

David Sabatini, Kuang Shen

Article

Shen et al J Biol Chem. 2019 Feb 22;294(8):2970-297

Insert

Nprl2 (NPRL2 Human)

Tags

HA

Expression

Mammalian

Mutation

R78A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GFP-recipient

Plasmid

#11655

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pARS3_MUbCAS9_GW

Plasmid

#128646

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

Depositor

G. Eric Schaller

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Tags

HA

Expression

Plant

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP49-2D(sfGFP)

Plasmid

#126967

Purpose

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP49-2C(sfGFP)

Plasmid

#126966

Purpose

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP49-2B(sfGFP)

Plasmid

#126965

Purpose

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-NT

Plasmid

#164857

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-NT

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET28c-Ku

Plasmid

#161936

Purpose

Expresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purification

Depositor

Harris Wang

Article

Yim et al ACS Synth Biol. 2020 Sep 29. doi: 10.102

Insert

ku (mku Mycobacterium tuberculosis)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP49-2D'(sfGFP)

Plasmid

#126994

Purpose

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1D'(sfGFP)

Plasmid

#126981

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-Tsc1

Plasmid

#19955

Depositor

Yue Xiong

Article

Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

Insert

Tsc1 (Tuberous sclerosis 1) (TSC1 Human)

Tags

Myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-GluA2-scFv-mNeon-KDEL

Plasmid

#230057

Purpose

AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

Depositor

Matthew Kennedy

Insert

Anti-GluA2 single chain variable fragment (scFv)

Use

AAV

Tags

ER retrieval motif (KDEL) and mNeon

Expression

Mammalian

Promoter

human synapsin

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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