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5mARF17 pGreenII0229

Plasmid

#12071

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

5mARF17 (ARF17 Mustard Weed)

Use

Plant transformation via agrobacterium

Expression

Plant

Mutation

miR160-resistant version of ARF17 has five silent…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pZF-AAA

Plasmid

#127465

Purpose

coding sequence for wild type zinc finger ZF-AAA (ZiF268)

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-AAA (EGR1 Human)

Use

Cell-free transcription–translation

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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myc-Med4 pBacPAK8

Plasmid

#15364

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

Med4 (MED4 Human, N/A)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 L272A

Plasmid

#19850

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 L272A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 272 to Alanine

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pRK5 Flag RagC(GD)/RagB(CTD) chimera

Plasmid

#112774

Purpose

express Flag-RagC (G domain) fused with with RagB (C terminal domain)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (G domain) fused with RagB (C terminal domain) (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagB(GD)/RagC(CTD) chimera

Plasmid

#112773

Purpose

express Flag-RagB (Gdomain) fused with RagC (C terminal domain)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (G domain) fused with RagC (C terminal domain) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCin4-3xFLAG-MKL1 D444-630

Plasmid

#19840

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D444-630 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 444-630

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pET32b-MED19

Plasmid

#15438

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

His and Trx

Expression

Bacterial

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
5mARF17 pGreenII0129

Plasmid

#12081

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

5mARF17 (ARF17 Mustard Weed)

Use

Plant transformation via agrobacterium

Expression

Plant

Mutation

miR160-resistant version of ARF17 has five silent…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
Opto-GPR21

Plasmid

#106009

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR21; Opto-GPR21; A11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR21 (GPR21 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR1

Plasmid

#105999

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR1; Opto-GPR1; A1)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR1 (GPR1 Human, Bovine)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCin4-3xFLAG-MKL1 S449A

Plasmid

#19842

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S449A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 449 to Alanine

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_Gb2-K

Plasmid

#25764

Purpose

Entry vector with TRE promoter driving mouse G beta 2 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 miR-shRNA (Gnb2 Mouse)

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pET41a-MED30

Plasmid

#15431

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED30 (Med30 Mouse)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pSN30

Plasmid

#100115

Purpose

expresses a chimeric KLF4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-KLF4 fused to a 'strong' VP16 domain (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TmiR_G12-E

Plasmid

#25761

Purpose

Entry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-MED9

Plasmid

#15408

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

MED9 (Med9 Mouse)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET41a-MED31

Plasmid

#15437

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED31 (Med31 Mouse)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-Med9 pBacPAK8

Plasmid

#15363

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

Med9 (Med9 Mouse)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
His-FLAG-Med18 pBacPAK8

Plasmid

#15371

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med18 (MED18 Human)

Use

Baculovirus construction

Tags

FLAG and His

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only