We narrowed to 14,449 results for: RING

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pTriEX-Antenna-GDI Rac1

Plasmid

#83359

Purpose

Expression of Antenna-Rac1

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Rac1 (RAC1 Human)

Tags

FRET Antenna (mCerulean-cpVenus) and His

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-WDR5

Plasmid

#59969

Purpose

Bacterial Expression GST-WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 (WDR5 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
FKBP-beta3(702-1094)-GFP

Plasmid

#100734

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP3B1 (AP3B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

appendage and ear only

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pEBG-bZIPa

Plasmid

#12436

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

bZIP of C/EBPa (CEBPA Human)

Tags

GST

Expression

Mammalian

Mutation

pEBG vector is from Bruce Mayer, may be different…

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pY036_ATP1A1_G3_Array

Plasmid

#86619

Purpose

Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA

Use

CRISPR; Co-selection via nhej using ouabain

Tags

3xHA

Expression

Mammalian

Promoter

CBh

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-mir144~451 EF1Alpha-puro-T2A-BFP

Plasmid

#164793

Purpose

Express miR-144~451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144~451 (MIR451A, MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-beta2(616-951)-mRuby2

Plasmid

#100730

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and mRuby2

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2

Plasmid

#196526

Purpose

Entry vector for Gateway with Eps8L2

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2-FL (EPS8L2 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG IL13-CAR

Plasmid

#157742

Purpose

knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Chimeric antigen receptor targeting IL13

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)

Plasmid

#100742

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD8A (CD8A Human)

Tags

LOVpep and tagRFP657

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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PITX3-2A-eGFP-PGK-Puro

Plasmid

#31943

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

PITX3 (PITX3 Human)

Use

Targeting donor

Tags

eGFP

Promoter

-

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pCINeo-mCHMP5

Plasmid

#11771

Depositor

Sankar Ghosh

Article

Shim et al J Cell Biol. 2006 Mar 27. 172(7):1045-56

Insert

chromatin modifying protein 5 (Chmp5 Mouse)

Expression

Mammalian

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pQLL-Tc1-8B_DR[EF1α-copGFP]

Plasmid

#218327

Purpose

Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-IY HA-GBRPL2

Plasmid

#89298

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

HA and IRES-YFP

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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nSaCas9 I744 miniABEMax V82G

Plasmid

#135379

Purpose

Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 )

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSaCas9 I744 miniABEMax V82G

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2281

Plasmid

#111902

Purpose

ssAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple346 (NEFM Human)

Use

AAV

Promoter

Ple346 (NEFM MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

Plasmid

#212112

Purpose

Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

GABARAP(G116A)-mTagBFP2 (GABARAP Entacmaea quadricolor, Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

G116A (lipidation deficiency) of GABARAP

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 CA

Plasmid

#133264

Purpose

Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(G19V) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 G19V

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 DN

Plasmid

#133263

Purpose

Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(T24N) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 T24N

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔKinase gRNA resistant

Plasmid

#163411

Purpose

Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The kinase domain (202-500 aa) is deleted. gRNA-t…

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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