161,488 results

1. PB-TO-NFIA.1-SOX9 (bi-directional promoter)

   Plasmid

   #182306

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   NFIA (NFIA Human)
   SOX9 (SOX9 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Piggybac

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-Bi-directional

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pet28a-His6-Halo-TEV-Nrf2

   Plasmid

   #62455

   Purpose

   Halo-His6 tagged Nrf2 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Nrf2 (NFE2L2 Human)
   

   Use

   Tags

   His6-Halo-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV6-mPER2-luc

   Plasmid

   #212035

   Purpose

   Expression of murine Per2-luciferase in mammalian cells

   Depositor

   David Hazlerigg

   Article

   Appenroth et al J Biol Rhythms. 2024 Dec;39(6):554-567.

   Insert

   mPer2-luciferase (Per2 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. yWL234 MCP-GFP-PABPC1*

   Plasmid

   #197054

   Purpose

   pUBC-NLS-HA-2xMCP-1xGFP-PABPC1*

   Depositor

   Robert Singer

   Article

   Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

   Insert

   pubc-nls-ha-MCP-GFP-PABPC1*
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ubc

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-CDH5-mRuby3-IRES-puro

   Plasmid

   #85144

   Purpose

   lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-20nmEML-mRFP

   Plasmid

   #231181

   Purpose

   Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 20 nm from a lentiviral vector

   Depositor

   Dmitry Lim

   Article

   Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

   Insert

   20nmEML-mRFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. cytoplasmic cpVenus control

   Plasmid

   #186788

   Purpose

   Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   cytoplasmic cpVenus control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pVC-FUS-R521C

   Plasmid

   #175067

   Purpose

   expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   FUS glycine rich protein (FUS Human)
   

   Use

   Tags

   HA and mVenus + mCherry

   Expression

   Mammalian

   Mutation

   changed Arginin 521 to Cystein; mVenus: C-Termina…

   Promoter

   CMV Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJ404-DogCatcher-sfGFP

   Plasmid

   #171930

   Purpose

   Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   DogCatcher-sfGFP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 6xHis-TagRFP-T_A1aY1

    Plasmid

    #158754

    Purpose

    Expression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_TagRFP-T_A1aY1
    

    Use

    Tags

    6x-Histidine tag and TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge