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177,697 results

Showing: 11261 - 11280 of 177697 results

  10. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    138566-plasmid-map-sequence-id-274798

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  11. pH-nCas9-PPE

    Plasmid
    #140447
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Publication
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140447-plasmid-map-sequence-id-296176

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  17. pCMV-Sport6-CD9-pHluorin

    Plasmid
    #130905
    Purpose
    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.
    Depositor
    DM Pegtel
    Publication
    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD9-pHluorin (CD9 Aequorea victoria, Human)
    Tags
    pHluorin
    Expression
    Mammalian
    Mutation
    phluorin inserted between Asn-50 and Asn-51 in ex…
    Promoter
    CMV
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    130905-plasmid-map-sequence-id-272542

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  19. pAAVS1-TRE-MA4-GFP

    Plasmid
    #114380
    Purpose
    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.
    Depositor
    Yuet Wai Kan
    Publication
    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MLL-AF4 fusion gene (AFF1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    TRE
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    114380-plasmid-map-sequence-id-220669

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Showing: 11261 - 11280 of 177697 results