We narrowed to 59,466 results for: %s

1. pNICKclos1.0

   Plasmid

   #73639

   Purpose

   Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   Cas9 nickase
   sGRNA to pyrE
   

   Use

   E.coli-clostridium shuttle vector

   Mutation

   D10A

   Promoter

   j23119 and ptb

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1delBRCT

   Plasmid

   #173938

   Purpose

   Bacterial expression of PARP1 lacking BRCT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delBRCT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 374-520; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA GFP10-RHOA

   Plasmid

   #133068

   Purpose

   Expresses RHOA GTPase with tripartite split GFP10

   Depositor

   Stéphanie Cabantous

   Article

   Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

   Insert

   ras homolog family member A (RHOA Human)
   

   Tags

   GFP10 (split-GFP tripartite)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQFlag-USP7 CS puroR

   Plasmid

   #46752

   Purpose

   Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C223S--catalytically inactive

   Promoter

   CMV

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEL063

   Plasmid

   #124895

   Purpose

   For genome editing in plant by SpCas9 variant(SpCas9-NG)

   Depositor

   Yong Zhang

   Article

   Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

   Plasmid

   #165456

   Purpose

   Inducible ZEB2 expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   ZEB2 (ZEB2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-neo-PP1-Alpha-H248K

   Plasmid

   #171268

   Purpose

   Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   PPP1CA-His248Lys (H248K)

   Promoter

   CMV

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX SH3(2)-4R

   Plasmid

   #112090

   Purpose

   Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-4R (NCK1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. TC1681

   Plasmid

   #164872

   Purpose

   CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A fused to PspCas13b (APOBEC3A )
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2T-cmv-SpCas9-TadCBEd-V106W-BlastR

    Plasmid

    #193849

    Purpose

    Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd-V106W
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-H1-shScramble-hsynapsin-EGFP

    Plasmid

    #220795

    Purpose

    control shRNA plasmid with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

    Plasmid

    #194279

    Purpose

    Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized VP64 dSaCas9 VP64 T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hU6 and hUbC

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-R2Tg-3xFlag

    Plasmid

    #223250

    Purpose

    Mammalian expression of C-terminally Flag-tagged R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEL066

    Plasmid

    #124897

    Purpose

    For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor

    Depositor

    Yong Zhang

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Integrin beta5-2XEGFP

    Plasmid

    #139779

    Purpose

    Detecting Integrin beta5 by fluorescence microscopy

    Depositor

    Staffan Stromblad

    Article

    Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

    Insert

    Integrin subunit beta5 (ITGB5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMCherry F8-2

    Plasmid

    #186034

    Purpose

    Express VHH F8-2 as a mCherry fusion in mammalian cells

    Depositor

    Isabelle Landrieu

    Article

    Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

    Insert

    VHH F8-2
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-EF1a-Cas9GFP-W

    Plasmid

    #200100

    Purpose

    Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMAL-C2X dAmp/Kana+

    Plasmid

    #154352

    Purpose

    Empty backbone vector

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-Tet-Puro-shIAPEz_2

    Plasmid

    #185017

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_IAPEz
    

    Use

    Lentiviral

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-Tet-Puro-shIAPEz_1

    Plasmid

    #185016

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_IAPEz
    

    Use

    Lentiviral

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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