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FnoCas12a-DR-cr in PBCSK-

Plasmid

#126642

Purpose

Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494)

Plasmid

#160577

Purpose

35S promoter sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31 PB (attP:TMtb:P35S:attB)

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-hMED9

Plasmid

#15365

Depositor

Joan Conaway, Ronald Conaway

Article

Paoletti et al Proc Natl Acad Sci U S A. 2006 Dec 12. 1

Insert

hMED9 (MED9 Human)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
HEL binding scFv D44.1

Plasmid

#111718

Purpose

Yeast Surface Display of D44.1 as a reference starting point for design

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

D44.1

Tags

cmyc tag

Expression

Yeast

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pVL94 - pOp4:VND7

Plasmid

#116016

Purpose

destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR

Expression

Plant

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-EKAR

Plasmid

#181858

Purpose

Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-EKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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49535-sgFMR1_E3B

Plasmid

#157783

Purpose

Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MO70-hJunctate - EF-mutant

Plasmid

#79597

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

Insert

junctate (ASPH Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

77DAD79 to 77AAA79

Promoter

CMV

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
dmBACCS2VL-IRES-dOrai-IRES-GFP

Plasmid

#72897

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-GFP

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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L309-myc-seipin-CT

Plasmid

#64063

Purpose

myc-tagged murine seipin (cytosolic C-terminus); lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

Insert

bscl2 (Bscl2 Mouse)

Use

Lentiviral

Tags

myc epitope

Expression

Mammalian

Mutation

Contains Arg326 to Ser443

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
LjUBQ:MtGLV6:T35s

Plasmid

#164741

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Article

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr3g095180.1

Tags

Terminator 35S

Expression

Plant

Promoter

Lotus japonicus Ubiquitin

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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LjUBQ:MtGLV2:T35s

Plasmid

#164740

Purpose

Overexpression of Peptide coding gene (Binary vector)

Depositor

Wolf Scheible

Article

Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

Insert

Medtr5g012400.1

Tags

Terminator 35S

Expression

Plant

Promoter

Lotus japonicus Ubiquitin

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-RG-mycStrep

Plasmid

#31840

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R95G

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
ShaCas9

Plasmid

#163794

Purpose

Expresses ShaCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pUDE714

Plasmid

#103021

Purpose

expression of a Cpf1 programming crRNA targetting HIS4 (crHIS4-4.S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crHIS4-4.S (HIS4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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CDK4-luciferase active (880)

Plasmid

#86657

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

867 base pairs including up to coding nucleotide …

Promoter

CDK5

Available Since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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4E2H.210_LUMABS

Plasmid

#155209

Purpose

Expresses 4E2H.210_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210_LUMABS

Tags

6x His Tag

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Barkor (∂CC)

Plasmid

#24404

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of amino acids 91-180 of the coiled-coil…

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only
49535-sgFMR1_E17A

Plasmid

#157782

Purpose

Expresses a sgRNA targeting stop codon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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