We narrowed to 12,204 results for: 112

1. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

   Plasmid

   #140000

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe 3XFLAG-wtGATA6-3XAU1 puro

   Plasmid

   #72607

   Purpose

   Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Retroviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX462-hPRKAA2-gRNA_A

   Plasmid

   #74376

   Purpose

   gRNA_A to knockout human AMPK alpha 2 using Cas9n.

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN_TT 3XFLAG-wtGATA6-3XAU1

   Plasmid

   #72612

   Purpose

   Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3XAU1 and 3XFLAG

   Expression

   Bacterial

   Promoter

   TRE tight promoter

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX462-hPRKAA2-gRNA_B

   Plasmid

   #74377

   Purpose

   gRNA_B to knockout human AMPK alpha 2 using Cas9n

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-BG505.SOSIP-QES.i03.c01-8his

   Plasmid

   #111846

   Purpose

   Mammalian expression plasmid for soluble BG505 SOSIP.664; QES mutant with enhanced binding to PG16

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Tags

   8his purification tag and CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p663-UBC-miniTurbo-V5-NRF2

   Plasmid

   #207811

   Purpose

   N-terminal tagged NRF2 for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   miniTurbo-V5-NRF2 (NFE2L2 Human)
   

   Use

   Lentiviral

   Tags

   miniTurbo-V5

   Promoter

   UBC

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p667-UBC-NRF2-V5-miniTurbo

    Plasmid

    #207812

    Purpose

    C-terminal tagged NRF2 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    NRF2-V5-miniTurbo (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Promoter

    UBC

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR - EGFP sgRNA 3

    Plasmid

    #51762

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRRLNeo-pEF1a-p53ashL344A-mCerulean

    Plasmid

    #69580

    Purpose

    Expresses p53-L344A mutant tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. ZNF9

    Plasmid

    #156152

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    ZNF9 (CNBP Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRIP-CMV-mtagBFP2-2A-FLAG-cGAS E225A/D227A

    Plasmid

    #102604

    Purpose

    Lentivector to express Flag-tagged and catalytically-inactive cGAS and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    E225A-D227A

    Promoter

    CMV

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti.PGK.blast-Flag-MFF-WT

    Plasmid

    #74379

    Purpose

    Lentiviral expression vector containing flag tagged WT MFF isoform 5

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pLenti GW V5 Eco/Dam mse LaminA

    Plasmid

    #182674

    Purpose

    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.

    Depositor

    Bas van Steensel

    Article

    Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:

    Insert

    Lamin A (Lmna Mouse)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Promoter

    HSP

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. plentiCRISPv2_FDX1-2

    Plasmid

    #184489

    Purpose

    Lentivirus all in one CRISPR vector targeting human FDX1 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLC-BFP-FADD

    Plasmid

    #75166

    Purpose

    LentiCRISPR-BFP with sgRNA targeting human FADD

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    FADD sgRNA (FADD Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-GAL4-IL35-IRES-mCherry-PGK-BFP

    Plasmid

    #223210

    Purpose

    Lentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL35 (IL12A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP

    Plasmid

    #223208

    Purpose

    Lentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    PDL1 (CD274 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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