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pLEX_307_ms2_VPR

Plasmid

#79376

Purpose

Recruits VPR to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VPR

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCMU-AUTr

Plasmid

#61204

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMT3928

Plasmid

#113331

Purpose

control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Inserts

beta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase

Use

Unspecified

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSBDEST.B

Plasmid

#79460

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Blasticidin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLCS108

Plasmid

#209329

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mCherry fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Sox17 GFP

Plasmid

#23334

Depositor

Sean Morrison

Article

Kim et al Cell. 2007 Aug 10. 130(3):470-83.

Insert

Sex determining region Y-box containing gene 17 (Sox17 Mouse)

Use

Mouse Targeting

Mutation

None

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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sfTq2-N1

Plasmid

#117931

Purpose

Superfolder mTurquoise2 (pNM112)

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2

Expression

Mammalian

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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SAFB1-pcDNA3.1

Plasmid

#32742

Depositor

Steffi Oesterreich

Article

Townson et al J Biol Chem. 2003 May 30;278(22):20059-6

Insert

SAFB1 (SAFB Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA6-HA-AML1-ETO

Plasmid

#12508

Depositor

Dong-Er Zhang

Article

Peterson et al Blood. 2007 May 15;109(10):4392-8. Epub

Insert

HA-AML1-ETO (RUNX1T1 Human)

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
Cenp-B INCENP GFP

Plasmid

#45238

Purpose

Target INCENP to centromeres via full length CENP-B

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B, INCENP-Δcen, GFP

Tags

GFP and full length CENP-B

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL1P1_crU626

Plasmid

#191776

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_p65_hsf1

Plasmid

#79374

Purpose

Recruits p65_hsf1 to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-p65-hsf1

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDB007ns2a

Plasmid

#99214

Purpose

Accessory plasmid for PACE of PylRS variants; negative selection

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp, PproK, and Ptet

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pDD364

Plasmid

#91831

Purpose

Lox511I-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox511I-SEC-Lox511I

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LaM8-AK74

Plasmid

#159594

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at AK74

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDD382

Plasmid

#91830

Purpose

Lox2272-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox2272-SEC-Lox2272

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pHelper_TetR/pTet-Tnpase (carbR)

Plasmid

#213911

Purpose

ATc-inducible himar1C9 transposase expression - carb/amp resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

TetR/ptet ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-ro2GFP

Plasmid

#82370

Purpose

Expression of redox sensitive GFP in mammalian cells

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Expression

Mammalian

Mutation

C48S/F64L/S147C/Q204C

Promoter

CMV

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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