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Showing: 11261 - 11280 of 28485 results

  2. pGL3-Basic-ZC3H12D_PromoterMUT

    Plasmid
    #153067
    Purpose
    ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter
    Depositor
    Silvia Monticelli
    Article
    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi
    Insert
    ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Mutagenesis to disrupt 3 BHLHE4040 binding sites.…
    Promoter
    None
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    153067-plasmid-map-sequence-id-304643

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  5. Desmoplakin I no force control (F40-based)

    Plasmid
    #118726
    Purpose
    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I (1-1952)-[YPet(short)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1952
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118726-plasmid-map-sequence-id-230443

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  8. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available Since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  12. pFUSEss-CHIg-mM-Arg210Ala_M18

    Plasmid
    #91734
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg210Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Arg210 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    91734-plasmid-map-sequence-id-194569

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  13. pFUSEss-CHIg-mM-Asn209Arg_M18

    Plasmid
    #91740
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Arg (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Arg (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    91740-plasmid-map-sequence-id-191795

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  15. pLgw EcoDam-V5-NKX2-5Y191C

    Plasmid
    #98598
    Purpose
    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning
    Depositor
    Richard Harvey
    Article
    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.
    Insert
    NKX2-5 (Nkx2-5 Mouse)
    Use
    Lentiviral; Damid
    Tags
    Dam (DNA adenine methyltransferase) and V5
    Expression
    Mammalian
    Mutation
    Y191C point mutant
    Promoter
    Heat Shock Minimal Promoter
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98598-plasmid-map-sequence-id-190106

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  16. pFUSEss-CHIg-mM-Asn209Ile_M18

    Plasmid
    #91746
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ile (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ile (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    91746-plasmid-map-sequence-id-190060

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  17. pFUSEss-CHIg-mM-Asn209Val_M18

    Plasmid
    #91756
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Val (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Val (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    91756-plasmid-map-sequence-id-190059

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  18. pLgw NKX2-5Y191C-V5-EcoDam

    Plasmid
    #98599
    Purpose
    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning
    Depositor
    Richard Harvey
    Article
    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.
    Insert
    NKX2-5 (Nkx2-5 Mouse)
    Use
    Lentiviral; Damid
    Tags
    V5 and Dam (DNA adenine methyltransferase)
    Expression
    Mammalian
    Mutation
    Y191C point mutant
    Promoter
    Heat Shock Minimal Promoter
    Available Since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    98599-plasmid-map-sequence-id-189181

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  19. pFUSEss-CHIg-mM-Asn209Tyr_M18

    Plasmid
    #91755
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Tyr (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Tyr (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91755-plasmid-map-sequence-id-182657

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  20. pFUSEss-CHIg-mM-Asn209Thr_M18

    Plasmid
    #91753
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Thr (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Thr (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91753-plasmid-map-sequence-id-182656

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Showing: 11261 - 11280 of 28485 results