159,530 results

1. pSUC01

   Plasmid

   #177204

   Purpose

   chromogenic reporter for gene knockout in Streptomyces

   Depositor

   Wenjun Guan

   Article

   Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

   Insert

   SaindC
   

   Use

   Synthetic Biology

   Tags

   NONE

   Expression

   Bacterial

   Mutation

   NONE

   Promoter

   TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. fwYellow chromoprotein

   Plasmid

   #117841

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, fwYellow
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMB52/14-3-3_1_247

   Plasmid

   #40541

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   YWHAG (YWHAG Human)
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Mutation

   WT; aa1-247

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. AAV pCAG-FLEX-EGFP-WPRE

   Plasmid

   #51502

   Purpose

   Can be used to generate AAV virus that will express EGFP in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVSV eGFP RABV-G

   Plasmid

   #31833

   Depositor

   Connie Cepko

   Article

   Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   VSV genome
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. mPA-GFP-Calnexin-N-14

   Plasmid

   #57122

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   I474N in Calnexin

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pB-mTSPAN4-GFP

   Plasmid

   #210329

   Purpose

   Overexpression of mTSPAN4

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   mTSPAN4 (Tspan4 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-mAirn-R-loop

   Plasmid

   #134900

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   mAirn promoter (Airn Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB1C3-Lux

   Plasmid

   #109383

   Purpose

   Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pARABAD-LUX operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ga13_2-mTq2

    Plasmid

    #112933

    Purpose

    Human Galpha13 tagged with mTurquoise, inserted at residue 128

    Depositor

    Dorus Gadella

    Article

    Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

    Insert

    Galpha13
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTOPO-MCL1

    Plasmid

    #21605

    Depositor

    Ulrich Maurer

    Article

    Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

    Insert

    MCL-1 (MCL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert ends with a STOP codon, no V5-His-tag.

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

12. tph-1(short)::rpl-22-HA

    Plasmid

    #122203

    Purpose

    C. elegans tagged ribosome expression plasmid

    Depositor

    Steven Flavell

    Article

    Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

    Insert

    tph-1(short)::rpl-22-HA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. mPA-GFP-Farnesyl-5

    Plasmid

    #57133

    Purpose

    Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. nLuc-PTHR1

    Plasmid

    #162577

    Purpose

    Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    Parathyroid Hormone Receptor 1 (PTH1R Human)
    

    Use

    Tags

    Nanoluc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-EGFP-Cdc42-Q61L

    Plasmid

    #12986

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. p0521s

    Plasmid

    #62862

    Purpose

    Episome vector for Phaeodactylum tricornutum (pCC1BAC-based)

    Depositor

    Hamilton Smith

    Article

    Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Episomal vector for phaeodacty…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)

    Plasmid

    #214880

    Purpose

    Lentiviral vector encoding RfxCas13d targeting CD46 guide

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crCD46-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC_N_ETV4

    Plasmid

    #167753

    Purpose

    Expresses ETV4 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ETV4 (ETV4 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ARTX_ctrl

    Plasmid

    #214065

    Purpose

    Fluorescent reporter system, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV.5’-LMNA-eGFP-KI

    Plasmid

    #170545

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for LMNA knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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