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px330 WAPL gRNA

Plasmid

#165595

Purpose

Insertion of WAPL degrom

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

WAPL (Wapl Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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px330 BRM gRNA

Plasmid

#165586

Purpose

Insertion of BRM degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

BRM (Smarca4 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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GP821

Plasmid

#164552

Purpose

Expresses Smo-FLAG K434R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMin-DHS-4D-GFP

Plasmid

#159653

Purpose

Reporter plasmid for the DHS-4D Otx2 enhancer element.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

DHS-4D

Expression

Mammalian

Promoter

TATA

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B

Plasmid

#163907

Purpose

Full length mouse KIF13B for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B 1-584

Plasmid

#163908

Purpose

Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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mWasabi-ADAP1

Plasmid

#163906

Purpose

Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

mWasabi fluorescent tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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GP758

Plasmid

#164541

Purpose

Expresses Sstr3-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Somatostatin receptor type 3 (Sstr3 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

WT

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Talin1-FERM

Plasmid

#110563

Purpose

bacterial expression of Talin 1

Depositor

Tina Izard

Insert

Talin 1 (Tln1 Mouse)

Tags

(His)8

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GP826

Plasmid

#164551

Purpose

Expresses Smo-FLAG K444, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GP825

Plasmid

#164550

Purpose

Expresses Smo-FLAG K434, 448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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GP823

Plasmid

#164554

Purpose

Expresses Smo-FLAG K448R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K448R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GP822

Plasmid

#164553

Purpose

Expresses Smo-FLAG K444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

One of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GP824

Plasmid

#164549

Purpose

Expresses Smo-FLAG K434, 444R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K434, 444R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

Two of the three Lysines in loop three of Smoothe…

Promoter

GgCryD1

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PD30

Plasmid

#164543

Purpose

Expresses Drd1-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Dopamine receptor D1 (Drd1 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP940

Plasmid

#164539

Purpose

Expresses SmoPi-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened R455A (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

R455A

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP938

Plasmid

#164538

Purpose

Expresses SmoPi-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened R455A (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

R455A

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP807

Plasmid

#164535

Purpose

Expresses Smo noK-FLAG-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG_Ub noK

Mutation

All 21 cytoplasmic Lysines of Smoothened and all…

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP806

Plasmid

#164534

Purpose

Expresses Smo noK-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Mutation

All 21 cytoplasmic Lysines of Smoothened changed …

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PD19

Plasmid

#164528

Purpose

Expresses Flag-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin noK

Use

Lentiviral

Tags

FLAG and Ub noK

Mutation

All seven Lysines of Ubiquitin changed to Arginine

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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