We narrowed to 12,334 results for: pcDNA

Showing: 11281 - 11300 of 12334 results

Results per page:

Sort results by:

pRD190

Plasmid

#163100

Purpose

Expression of mEos3.2 in eukaryotes to stain the nuclear compartment

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mEos3.2_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKK-TEV-FLAG

Plasmid

#105783

Purpose

Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-FLAG

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE3987

Plasmid

#137716

Purpose

Expresses dLbCas12a-RVRR-BE in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

dLbCas12a-RVRR-BE

Tags

6xHis, APOBEC-1, SV40 NLS, and UGI

Expression

Mammalian

Mutation

G532A, K538V, Y542R, K595R

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hMEF2A K403R

Plasmid

#118355

Purpose

This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K403R

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmyc-GFP-TNRC6A-NLS/NES-mut

Plasmid

#42002

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pIKAT111

Plasmid

#69996

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

C1459T (R487C)

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRR.6

Plasmid

#69997

Purpose

Mammalian expression of cytochrome p450 CYP2B6 variant

Depositor

Kathrin Klein

Article

Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

Insert

CYP2B6 (CYP2B6 Human)

Expression

Mammalian

Mutation

Q172H, K262R

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
FR_HNF1B_A263insGG

Plasmid

#31103

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

mutation A263insGG

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SENP2 C548A

Plasmid

#126592

Purpose

Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C548A

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
C211

Plasmid

#174360

Purpose

For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (PABPC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
B9L-BirA*

Plasmid

#109146

Purpose

To generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

Depositor

Mariann Bienz

Article

van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

Insert

B9L (Bcl9l Mouse)

Tags

BirA(R118G) and Flag

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
FR_HNF1B_P328L329del

Plasmid

#31102

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

mutation P328L329delCCTCT

Available Since

Oct. 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRD397

Plasmid

#163104

Purpose

Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mTurquoise2_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CNC3G (CASANOVA-C3 with glycine linkers)

Plasmid

#137192

Purpose

Expresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory protein

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

Insert

CNC3G (CASANOVA-C3 with glycine linkers)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
1029-AIDx-CP

Plasmid

#135341

Purpose

Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

CP 1029 nSpyCas9-hAIDx

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR173

Plasmid

#106058

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR173; Opto-GPR173; E12)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR173 (GPR173 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR31

Plasmid

#106014

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR31; Opto-GPR31; B4)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR31 (GPR31 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SENP2 WT

Plasmid

#126591

Purpose

Expresses siRNA resistant SENP2 (WT) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant to GAGGAGAUAUUCAGACAUU

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAL4-DBD-TBX1wt-IDR

Plasmid

#145254

Purpose

Expresses fusion of GAL4 DNA-binding domain and TBX1wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBX1wt-IDR (TBX1 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-GPR25

Plasmid

#106011

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR25; Opto-GPR25; B1)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR25 (GPR25 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge