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pRK5_mCherry-SURF6

Plasmid

#238252

Purpose

For overexpression of mCherry-SURF6

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-SURF6

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG

Plasmid

#238296

Purpose

For RNA injection of Nanog-mNG into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-KS-FtoG

Plasmid

#238267

Purpose

For overexpression of V5-nb-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_TagBFP-P2A

Plasmid

#238272

Purpose

For overexpression of TagBFP-control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TagBFP

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF5)-TS.FF5x1-bGH

Plasmid

#235277

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mGL-bGH

Plasmid

#235299

Purpose

mGreenLantern expression to serve as a transfection marker, EF1a-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF4)-TS.FF6x1-bGH

Plasmid

#235282

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miRE.FF5)-TS.FF6x1-bGH

Plasmid

#235260

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mGL-bGH

Plasmid

#235255

Purpose

mGreenLantern expression to serve as a transfection marker, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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hPGK-mRuby2-bGH

Plasmid

#235254

Purpose

ComMAND base gene regulating mRuby2, hPGK-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hPGK (human phosphoglycerate kinase)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF5)-TS.FF6x1-bGH

Plasmid

#235276

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235270

Purpose

ComMAND open-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235271

Purpose

ComMAND closed-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miR.FF4)-TS.FF4x1-bGH

Plasmid

#235283

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF4x1-mRuby2-bGH

Plasmid

#235274

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miRE.FF5)-TS.FF5x1-bGH

Plasmid

#235261

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-tagBFP-bGH

Plasmid

#235257

Purpose

tagBFP expression to serve as a "filler" plasmid for transfections

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

tagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

Silent mutations to remove BsaI sites in tagBFP

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EFS-mRuby2-bGH

Plasmid

#235253

Purpose

ComMAND base gene regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2-bGH

Plasmid

#235269

Purpose

ComMAND base gene regulating mRuby2, EF1a

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-TS.FF6x1-mRuby2-bGH

Plasmid

#235275

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (open-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge