We narrowed to 42,138 results for: TRO

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TC476

Plasmid

#122629

Purpose

Punc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Expression

Worm

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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TRMPV-Neo

Plasmid

#27990

Purpose

Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TRMPV-Neo (shRen)

Use

RNAi and Retroviral; Fluorescent reporters

Expression

Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pDS277

Plasmid

#34973

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

V529N

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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Endoplasmic reticulum-targeted optodPLD

Plasmid

#140059

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sac1(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sac1(TM), and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-N-GFP

Plasmid

#172974

Purpose

MAC-tagged GFP expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GFP

Tags

MAC-tag

Expression

Bacterial and Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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TRMPV-Hygro

Plasmid

#27996

Purpose

Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TRMPV-Hygro (shLuci-1309)

Use

RNAi and Retroviral; Fluorescent reporters

Expression

Mammalian

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mTagBFP-T2A-TetOn3G

Plasmid

#179888

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

mTagBFP

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_213

Plasmid

#133456

Purpose

H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

Control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1- Ncad-GFP

Plasmid

#35676

Depositor

James Jontes

Article

Biswas et al J Cell Biol. 2010 Nov 29;191(5):1029-41.

Insert

Ncad (cdh2 Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-MKRN1

Plasmid

#78751

Purpose

To overexpress MKRN1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMK401 (Hygro-P2A-mAID-Nluc)

Plasmid

#121196

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-Nluc

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-zz-p15_O

Plasmid

#147110

Purpose

Mammalian Expression of zz-p15

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

zz-p15 (NXT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-ninein coiled-coil 2

Plasmid

#73522

Purpose

Encodes the second coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 2 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-ninein coiled-coil 1

Plasmid

#73521

Purpose

Encodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 1 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
mEm-CoilE-Actin

Plasmid

#181993

Purpose

CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

mEm-CoilE-Actin

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-C1V1-TT-mRuby2-ST

Plasmid

#109124

Purpose

Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

C1V1-TT-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX3x-hRanBP2-(IR1+M)

Plasmid

#53140

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2631-2711)

Promoter

tac

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW

Plasmid

#133359

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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mVenus-RapR-PTPPest

Plasmid

#188657

Purpose

Rapamycin regulated PTPPest with mVenus tag

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Protein Tyrosine Phosphatase Pest (PTPN12 )

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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LP377

Plasmid

#90201

Purpose

pCMV-myc with cDNA encoding HsTRAPPC11 aa residues 701-1133

Depositor

Lotte Pedersen

Article

Schou et al Cilia. 2014 Jun 18;3:6. doi: 10.1186/204

Insert

TRAPPC11 (TRAPPC11 Human)

Tags

Myc

Expression

Mammalian

Mutation

aa residues 701-1133

Promoter

CMV

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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