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pLV-EF1a-H2B-miRFP670

Plasmid

#212668

Purpose

Express tagged H2B

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

H2B (H2BC11 Human)

Use

Lentiviral

Tags

miRFP670

Promoter

EF-1a

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1Mut-T2A-tdTomato

Plasmid

#60644

Purpose

Expresses Kir2.1Mut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kcnj2 (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

GYG to AAA (aa144-146)

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-Nesprin-1αKASH

Plasmid

#187015

Purpose

Disruption of LINC Complex

Depositor

Jan Lammerding

Article

Lombardi et al J Biol Chem. 2011 Jul 29;286(30):26743-5

Insert

Nesprin-1alphaKASH

Use

Lentiviral

Tags

mCherry

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Salsa-BC1-P1A1

Plasmid

#202842

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC1

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TPC1-G-GECO1.2

Plasmid

#207143

Purpose

Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPCN1 (TPCN1 Human)

Tags

G-GECO1.2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSiMPls_N + pSiMPls_C

Plasmid

#167203

Purpose

SiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycin

Depositor

Barbara Di Ventura

Article

Palanisamy et al ACS Omega. 2021 May 21;6(22):14148-14153

Inserts

EGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263

Expression

Bacterial

Promoter

AmpR, CAT, pBAD, and pTrc

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx5

Plasmid

#74344

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx5. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 5 (Dlx5 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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FKBP-C-TEV 219

Plasmid

#100096

Purpose

SNIPer plasmid, C terminal TEV fused to FKBP

Depositor

Jim Wells

Article

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

C-TEV

Use

Retroviral

Tags

FKBP and Myc

Expression

Mammalian

Mutation

C terminal domain

Promoter

CMV

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSL0283 (pTnsABC)

Plasmid

#130633

Purpose

Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTnsA, VchTnsB, VchTnsC

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

PcUBI4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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PNCA-GFP-dYY1

Plasmid

#67791

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

Insert

MMLV-LTR-GFP

Mutation

Deletion of LTR U3 YY1 binding domain

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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Multi-frame tag

Plasmid

#128610

Purpose

In the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.

Depositor

Tim Stasevich

Article

Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

Insert

MF tag

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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cmv-sfGFP-GB1-scAB-ubc-nls-pcp-10xSunTag(SRv1.1-2P)

Plasmid

#185801

Purpose

one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-sfGFP-GB1-scAB--ubc-nls-pcp-10xSunTag

Expression

Mammalian

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pNeuroD1-ERT2CreERT2

Plasmid

#237402

Purpose

Tamoxifen-dependent expression of ERT2CreERT2 in NeuroD1-positive cells

Depositor

Yusuke Kishi

Article

Sakai et al Development. 2025 Jul 1;152(13):dev20456

Insert

ERT2CreERT2

Expression

Mammalian

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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smHA multi-frame tag

Plasmid

#128611

Purpose

a spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.

Depositor

Tim Stasevich

Article

Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

Insert

smHA MF tag

Expression

Mammalian

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-RalGDS

Plasmid

#118315

Purpose

Expression of human RalGDS

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RalGDS (RALGDS Human)

Tags

GFP

Expression

Mammalian

Mutation

fragment of human RalGDS 203aa

Promoter

CMV

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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H2B-HT-emiRFP703

Plasmid

#183985

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

H2B (H2BC11 Human)

Tags

HaloTag, emiRFP703

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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